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The adsorption of proteins at interfaces plays a role in many ?elds, such as health, food, environment and analysis. Fundamental aspects are useful when considering applications. We focus here especially on solid-liquid interfaces and present a few fundamental studies regarding adsorption - netics and conformational changes, and examples of applications to sensors and membranes. The ?rst part is dedicated to fundamental studies performed using - tical waveguide lightmode spectroscopy, as an example of a technique that has the advantage of not requiring labelled proteins, but is limited to s- ci?c supports. Conversely, the radiolabelling of proteins, which has the disadvantage of any labelling process, allows application to any kind of s- faces. As proteins bear both positive and negative charges, we can expect thein?uenceofanelectric?eldnormaltothe interfaceonthe pack- ing order at interfaces. The re?ning of data treatment may also lead to the determination of useful structural parameters. The balance between protein-surface and protein-protein interactions is a key point for the - scription of the structure at high coverage of the surface. Electrokinetic methods, like measurement of the streaming potential, may be helpful in the electrical characterisation of the interfacial layer facing the solution. The second part includes different bench techniques that were dev- oped to improve the sensitivity of the characterisation of the orientation and structure of the proteins at interfaces: dual polarisation interferometry and total internal re?ection ellipsometry are such recent examples.

Adsorption --- Proteins --- Solid-liquid interfaces --- Protéines --- Interfaces solide-liquide --- EPUB-LIV-FT LIVCHIMI SPRINGER-B

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Electrocatalysis. --- Fuel cells. --- Solid-liquid interfaces. --- Electrocatalyse --- Piles à combustible --- Interfaces solide-liquide --- Relation between energy and economics --- Electrical engineering --- Electrochemistry --- Piles à combustible

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Provides a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. This book presents the methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS).

Adsorption --- Proteins --- Solid-liquid interfaces --- Protéines --- Interfaces solide-liquide --- Adsorption. --- Proteins. --- Solid-liquid interfaces. --- Amino Acids, Peptides, and Proteins --- Molecular Conformation --- Chemical Processes --- Physicochemical Processes --- Metabolism --- Chemical Phenomena --- Molecular Structure --- Physicochemical Phenomena --- Chemicals and Drugs --- Metabolic Phenomena --- Phenomena and Processes --- Biochemical Phenomena --- Protein Conformation --- Absorption --- Mechanical Engineering --- Chemical & Materials Engineering --- Engineering & Applied Sciences --- Bioengineering --- Chemical Engineering --- Protéines --- EPUB-LIV-FT LIVCHIMI SPRINGER-B --- Liquid-solid interfaces --- Proteids --- Sorption --- Chemistry. --- Biotechnology. --- Physical chemistry. --- Biochemistry. --- Biophysics. --- Biological physics. --- Materials --- Thin films. --- Biophysics and Biological Physics. --- Physical Chemistry. --- Biochemistry, general. --- Surfaces and Interfaces, Thin Films. --- Surfaces. --- Interfaces (Physical sciences) --- Biomolecules --- Polypeptides --- Proteomics --- Separation (Technology) --- Surface chemistry --- Chemistry, Physical organic. --- Surfaces (Physics). --- Biological and Medical Physics, Biophysics. --- Physics --- Surfaces (Technology) --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Chemical engineering --- Genetic engineering --- Composition --- Materials—Surfaces. --- Films, Thin --- Solid film --- Solid state electronics --- Solids --- Coatings --- Thick films --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Biological physics