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This is comprehensive overview of a vital area of scientific enquiry, which covers a broad spectrum of issues. With contributions from some of the key researchers in the field, Adult Stem Cells: Biology and Methods of Analysis offers readers a historical perspective as well as unique insights into cutting-edge thoughts. The volume contextualizes the recent discovery of stem/progenitor cell populations resident in many adult tissues and organs. It confronts the complexities scientists face in trying to validate these cells, while it also describes and critically evaluates the methods currently used to assess stem cell self-renewal. The chapters also seek to distinguish this process from other aspects of cell survival, such as the regulation of life span, senescence, and immortalization at a molecular level. The monograph begins with a section that examine the basic biology of adult stem cells, including chapters on the emerging role of microRNAs in regulating their fate and the molecular mechanisms that govern their self-renewal, the book moves on to analyze the varying methodologies employed in characterizing these elusive elements of our genetic make-up. The second section details in-vivo lineage tracing of tissue-specific stem cells, explores the neural stem cell paradigm, and considers the function of ABC transporters and aldehyde dehydrogenase in adult stem-cell biology. The final section shifts the focus to the life-span regulation and immortalization and features a chapter on the cancer stem cell paradigm. This is an authoritative volume on one of the frontiers of genetic research, and will serve as a valuable resource, not just for established scientists but also for those now entering the field of stem cell biology.

Adult stem cells. --- Adult stem cells --- Stem Cells --- Clinical Laboratory Techniques --- Investigative Techniques --- Cells --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anatomy --- Adult Stem Cells --- Cytological Techniques --- Biology --- Health & Biological Sciences --- Cytology --- Stem cells. --- Cytology. --- Cell biology --- Cellular biology --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Life sciences. --- Biotechnology. --- Cell biology. --- Life Sciences. --- Stem Cells. --- Cell Biology. --- Cytologists --- Chemical engineering --- Genetic engineering

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Multipotent Stem Cells --- MSCs --- Adult Stem Cells --- cancer-associated MSCs --- Mesenchymal Stromal Cells --- Multipotent cell differentiation --- Bone Marrow Transplantation --- nestin --- congenital heart disease --- in vivo MSC

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The aim of volume 7 of Human Cell Culture is to provide clear and precise methods for growing primary cultures of adult stem cells from various human tissues and describe culture conditions in which these adult stem cells differentiate along their respective lineages. The book will be of value to biomedical scientists and of special interest to stem cell biologists and tissue engineers. Each chapter is written by experts actively involved in growing human adult stem cells.

Adult stem cells. --- Adult stem cells --- Publication Formats --- Cell Physiological Processes --- Clinical Laboratory Techniques --- Stem Cells --- Cells --- Culture Techniques --- Investigative Techniques --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anatomy --- Cell Physiological Phenomena --- Phenomena and Processes --- Cytological Techniques --- Cell Differentiation --- Methods --- Cells, Cultured --- Cell Culture Techniques --- Laboratory Manuals --- Adult Stem Cells --- Biology --- Health & Biological Sciences --- Cytology --- Stem cells. --- Cell culture. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Medicine. --- Biomedical engineering. --- Biomedicine. --- Biomedicine general. --- Biomedical Engineering. --- Cultures (Biology) --- Technique --- Biomedical Engineering and Bioengineering. --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Biomedicine, general. --- Health Workforce

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This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook? and handy illustrations. The collection of protocols includes the isolation and maintenance of ste

Adult Stem Cells. --- Cell differentiation. --- Physiology. --- Stem Cells. --- Stem Cells --- Cell Physiological Processes --- Cell Physiological Phenomena --- Cells --- Anatomy --- Phenomena and Processes --- Adult Stem Cells --- Cell Differentiation --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Stem cells --- Stem cells. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cell fate specification --- Cell specification --- Differentiation of cells --- Fate specification of cells --- Specification of cells --- Differentiation --- Fate specification --- Specification --- Morphogenesis

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Regenerative medicine and tissue engineering play significant roles in the treatment of currently intractable conditions, such as chronic heart failure, stroke, chronic osteoarthritis, and other maladies. Regenerative medicine and tissue engineering generally depend on the utilization of stem cells to treat patients but may also utilize mature cells that would not normally be considered as stem cells (e.g., skin). Stem cells (like mature cells) may be obtained from many sources in the body including bone marrow, cord blood, cord tissue, adipose tissue, etc. Although stem cells are often used in therapy immediately upon isolation, in many circumstances, the stem and progenitor cells will be harvested, processed and banked frozen until a later time. Biobanking is a convenient alternative to same-day therapeutic use, in that it allows for patient recovery (e.g., from liposuction), provides time to identify the best treatment options, and may allow for multiple interventions with additional patient inconvenience or risk.

Medicine. --- MSCs --- EVs --- exosomes --- tissue regeneration --- immunomodulation --- biobanking --- adult stem cells --- ground-state --- autologous transplantation --- regenerative medicine --- stem cell banking --- newborn stem cells --- perinatal stem cells --- umbilical cord tissue --- umbilical cord blood --- placenta --- Vascular tissue engineering --- human cord blood-derived endothelial cells --- endothelialization --- vascular graft --- Munich Vascular Biobank --- atherosclerosis --- human vascular tissue --- RIN --- RNA fragmentation --- adipose tissue --- cryopreservation --- autologous --- fat grafting --- cryogenic storage --- lipoaspirate --- cosmetic surgery --- MSCs --- EVs --- exosomes --- tissue regeneration --- immunomodulation --- biobanking --- adult stem cells --- ground-state --- autologous transplantation --- regenerative medicine --- stem cell banking --- newborn stem cells --- perinatal stem cells --- umbilical cord tissue --- umbilical cord blood --- placenta --- Vascular tissue engineering --- human cord blood-derived endothelial cells --- endothelialization --- vascular graft --- Munich Vascular Biobank --- atherosclerosis --- human vascular tissue --- RIN --- RNA fragmentation --- adipose tissue --- cryopreservation --- autologous --- fat grafting --- cryogenic storage --- lipoaspirate --- cosmetic surgery