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Receptors, Cannabinoid --- Luciferases

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The glomulin gene was recently identified in the laboratory where this work was done. Mutations in this gene are known to cause hereditary glomuvenous malformations. Previous studies have shown that glomulin could play a role in the differentiation of vascular smooth muscle cells. However, its exact function remains unknown.
In order to better understand the spatio-temporal expression of glomulin, that are conserved between different species. We also predicted a minimal promoter of 548 bp. The most interesting regions were cloned either upstream or downstream of a luciferase reporter gene, which allowed to measure their transcriptional activity in different cell types. Cells expressing glomulin endogenously were chosen as target, since they are more likely to have the appropriate transcription factors. Detailed analysis of the most important regions allowed us to identify some transcriptional factors potentially involved in the regulation of glomulin expression. In order to study this expression in vivo; we also created transgenic mice. LacZ reporter gene was cloned downstream of a 5 kb fragment of promoter, in frame with the ATG of glomulin. Assuming that this fragment is sufficient, it should reveal the expression profile of glomulin in mouse Le gène de la glomuline, dont les mutations causent les malformations glomuveineuses héréditaires, fût récemment identifié par le laboratoire au sein duquel ce travail a été réalisé. Des études antérieures ont démontré que la glomuline pourrait jouer un rôle dans la différenciation des cellules musculaires lisses vasculaires. Cependant, sa fonction exacte reste encore peu connue. Afin de mieux comprendre l’expression spatio-temporelle de la glomuline, il est donc nécessaire de caractériser la région promotrice du gène et de localiser les divers éléments régulateurs.
L’utilisation de plusieurs outils bio-informatiques nous a permis de mettre en évidence des régions du gène de la glomuline conservées entre différentes espèces ainsi que de prédire une région promotrice minimale de 548 pb. Les régions les plus intéressantes ont été clonées de part et d’autre d’un gène rapporteur, la luciférase, qui permet d’en mesurer l’activité transcriptionnelle dans plusieurs types cellulaires différents. Des cellules cibles exprimant naturellement la glomuline ont été choisies afin d’augmenter la probabilité qu’elles aient les facteurs de transcription adéquats. Une analyse approfondie des régions les plus importantes a permis d’identifier quelques facteurs de transcription potentiellement impliqués dans la régulation transcriptionnelle du gène de la glomuline. Afin d’étudier l’expression de la glomuline in vivo, nous avons également entamé la création de souris transgéniques. Le gène rapporteur LacZ, cloné en aval de 5 kb de région promotrice est en phase au niveau de l’ATG de la glomuline, devrait en révéler le profil d’expression, pour autant que ce fragment soit suffisant

Pericytes --- Muscle, Smooth, Vascular --- Luciferases --- Gene Expression --- Arteriovenous Malformations --- Mice, Transgenic

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Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels

Biological techniques --- Enzymology --- General biophysics --- Chemiluminescence --- Bioluminescence --- Luminescence --- Luciferases --- metabolism --- 577.15 --- Luminescent Measurements. --- Chemoluminescence --- Animal light --- Animal luminescence --- Light production in organisms --- Photobiology --- Bioluminescence Measurements --- Bioluminescent Assays --- Bioluminescent Measurements --- Chemiluminescence Measurements --- Chemiluminescent Assays --- Chemiluminescent Measurements --- Chemoluminescence Measurements --- Luminescence Measurements --- Luminescent Assays --- Luminescent Techniques --- Phosphorescence Measurements --- Phosphorescent Assays --- Phosphorescent Measurements --- Assay, Bioluminescent --- Assay, Chemiluminescent --- Assay, Luminescent --- Assay, Phosphorescent --- Assays, Bioluminescent --- Assays, Chemiluminescent --- Assays, Luminescent --- Assays, Phosphorescent --- Bioluminescence Measurement --- Bioluminescent Assay --- Bioluminescent Measurement --- Chemiluminescence Measurement --- Chemiluminescent Assay --- Chemiluminescent Measurement --- Chemoluminescence Measurement --- Luminescence Measurement --- Luminescent Assay --- Luminescent Measurement --- Luminescent Technique --- Measurement, Bioluminescence --- Measurement, Bioluminescent --- Measurement, Chemiluminescence --- Measurement, Chemiluminescent --- Measurement, Chemoluminescence --- Measurement, Luminescence --- Measurement, Luminescent --- Measurement, Phosphorescence --- Measurement, Phosphorescent --- Measurements, Bioluminescence --- Measurements, Bioluminescent --- Measurements, Chemiluminescence --- Measurements, Chemiluminescent --- Measurements, Chemoluminescence --- Measurements, Luminescence --- Measurements, Luminescent --- Measurements, Phosphorescence --- Measurements, Phosphorescent --- Phosphorescence Measurement --- Phosphorescent Assay --- Phosphorescent Measurement --- Technique, Luminescent --- Techniques, Luminescent --- Light --- Enzymes. Catalysts of biological reactions. Enzymology --- metabolism. --- Bioluminescence. --- Chemiluminescence. --- LUCIFERASE --- Luminescence. --- 577.15 Enzymes. Catalysts of biological reactions. Enzymology --- Luciferase --- Metabolism. --- Luminescent Measurements --- Chemiluminescentie. --- Chimioluminescence. --- Bioluminescentie. --- Luciferases - metabolism