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The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Meiosis: Volume 2, Cytological Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
Cytology. --- Microbiology. --- Cell Biology. --- Eukaryotic Microbiology. --- Microbial biology --- Biology --- Microorganisms --- Cell biology --- Cellular biology --- Cells --- Cytologists
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The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 1, Molecular and Genetic Methods details methods for culturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. Methods are provided for studies in yeasts, plants, and animals, including mouse and human. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Meiosis: Volume 1, Molecular and Genetic Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
Cytology. --- Microbiology. --- Cell Biology. --- Eukaryotic Microbiology. --- Microbial biology --- Biology --- Microorganisms --- Cell biology --- Cellular biology --- Cells --- Cytologists
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Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field. Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.
Microbiology. --- Gene expression. --- Eukaryotic Microbiology. --- Gene Expression. --- Microbial biology --- Biology --- Microorganisms --- Genes --- Genetic regulation --- Expression
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Symbioses and Stress centers on the question of how organisms in tight symbiotic associations cope with various types of abiotic and biotic stress. In its original sense, symbioses cover all kinds of interactions among unrelated organisms, whereas in a narrower concept, the term is often referred to as mutualism. Evolutionary biology recognizes symbiosis as an integrative process, and most fundamental evolutionary innovations arose from cooperative symbioses. Mutualisms contribute to stress tolerance, ecosystem stability, and evolutionary radiation of cooperating organisms. Modern eukaryotic cells are the result of the endosymbiotic union of prokaryotic ancestors as well as diverse exosymbiotic associations. This cooperative aggregation appears more successful than its independent parts. This new book presents functional and evolutionary aspects of mutually beneficial symbioses among unrelated organisms.
Life Sciences. --- Eukaryotic Microbiology. --- Microbiology. --- Evolutionary Biology. --- Freshwater & Marine Ecology. --- Life sciences. --- Aquatic biology. --- Evolution (Biology). --- Microbiology. --- Sciences de la vie --- Hydrobiologie --- Evolution (Biologie) --- Microbiologie
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This book emphasises the important role that protozoa play in many natural ecosystems. To shed new light on their individual adaptive skills, the respective chapters examine the ecology and functional biology of this diverse group of eukaryotic microbes. Protozoa are well-established model organisms that exemplify many general problems in population ecology and community ecology, as well as evolutionary biology. Their particular characteristics, like large population sizes, life cycles and motile sensory behaviour, have a profound impact on their survival, distribution, and interaction with other species. Thus, readers will also be introduced to protozoan habitats in a broad range of environments. Even though this group of unicellular organisms is highly diverse, the authors focus on shared ecological patterns. Students and scientists working in the areas of eukaryotic microbiology and ecology will appreciate this updated and revised 2nd Edition as a valuable reference guide to the “lifestyles” of protozoa. .
Microbial ecology. --- Microbiology. --- Aquatic ecology . --- Microbial Ecology. --- Eukaryotic Microbiology. --- Freshwater & Marine Ecology. --- Aquatic biology --- Ecology --- Microbial biology --- Biology --- Microorganisms --- Environmental microbiology --- Microbiology --- Protozoa. --- Protozoans --- Protozoons --- Invertebrates --- Protista --- Protozoology
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Dieses inspirierende und motivierende Lehrbuch zeichnet fächerübergreifend ein beeindruckendes Gesamtbild der biologischen Vielfalt. Spannende Aspekte der Evolution der Erde und des Lebens werden durch interdisziplinäre Verknüpfung geowissenschaftlicher und biowissenschaftlicher Aspekte aus einer ganz neuen Perspektive anschaulich vermittelt. Der Schwerpunkt liegt hierbei – unter Einbeziehung aktuellster wissenschaftlicher Erkenntnisse – auf dem Verständnis von Konzepten und Mechanismen. Dieses Buch richtet sich an Studierende der Bio- und Geowissenschaften und an alle an der Vielfalt des Lebens interessierten Leser. Das innovative Lehrbuchkonzept regt – gleichzeitig als Lesebuch, Bilderbuch und Lexikon der organismischen Biologie – sowohl Anfänger als auch Fortgeschrittene zu einem visuellen und intuitiven Lernen an. Jede Doppelseite bietet ein in sich geschlossenes, anschaulich bebildertes Kapitel mit themenbezogenem Glossar und weiterführenden Verweisen. Für Dozenten und Lehrer, für Schule und Hochschule bietet dieses Buch ein reichhaltiges Nachschlagewerk und einen umfassenden Fundus an didaktisch durchdachten und lernfreundlich illustrierten Lehrmaterialien. Der Inhalt reicht - von der Entstehung der Erde über die geowissenschaftlichen Rahmenbedingungen und die Verknüpfung zwischen biologischer und geologischer Evolution bis zur Entstehung des Menschen, - von grundlegenden Mechanismen der Entstehung und Erhaltung der Diversität bis zur globalen Verteilung der heutigen Biodiversität, - von den Anfängen der biologischen Systematik in der griechischen Philosophie und der Bibel über die darwinsche Evolution und die Struktur und Funktion von Arten und Organismen bis zu den modernen Erkenntnissen der Megasystematik und Phylogenie. Mit diesem einzigartigen Konzept verschafft dieses Lehrbuch einen soliden Überblick über die Erdgeschichte und Biodiversität. Die Verknüpfung der verschiedenen Fachdisziplinen fördert das Verständnis übergeordneter Prinzipien und naturwissenschaftlicher Zusammenhänge. Die Autoren: Prof. Dr. Jens Boenigk und Dr. Sabina Wodniok sind Dozenten für Biodiversität und Botanik an der Universität Duisburg-Essen.
Biodiversity. --- Evolutionary biology. --- Historical geology. --- Animal systematics. --- Animal taxonomy. --- Plant systematics. --- Plant taxonomy. --- Microbiology. --- Evolutionary Biology. --- Historical Geology. --- Animal Systematics/Taxonomy/Biogeography. --- Plant Systematics/Taxonomy/Biogeography. --- Eukaryotic Microbiology.
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This volume focuses on mitochondrial RNA metabolism, emphasizing recent discoveries and technological advances in this fast moving area that increase our understanding of mitochondrial gene function. Topics addressed include the interplay of mitochondria with the nucleus and cytosol, structure-function connections, and relevance to human disease. Mitochondria are the powerhouses of the cell, and a great deal is known about mitochondrial energy metabolism. Less well known is the plethora of amazing mechanisms that have evolved to control expression of mitochondrial genomes. Several RNA processes and machineries in protozoa, plants, flies and humans are discussed, including: transcription and RNA polymerase mechanism; tRNA processing of 5′ and 3′ ends; mRNA maturation by nucleotide insertion/deletion editing and by RNA splicing; mRNA stability; and RNA import. Specialized factors and ribonucleoproteins (RNPs) examined include pentatricopeptide repeat (PPR) proteins, RNase P, polymerases, helicases, nucleases, editing and repair enzymes. Remarkable features of these processes and factors are either not found outside mitochondria, differ substantially among eukaryotic lineages, or are unique in biology. .
Life sciences. --- Gene expression. --- Nucleic acids. --- Microbiology. --- Life Sciences. --- Nucleic Acid Chemistry. --- Gene Expression. --- Eukaryotic Microbiology. --- RNA --- Mitochondria. --- Metabolism. --- Chondriosomes --- Cell organelles --- Protoplasm --- Ribonucleic acid metabolism --- Microbial biology --- Biology --- Microorganisms --- Genes --- Genetic regulation --- Polynucleotides --- Biomolecules --- Expression
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With the development of fungal transformation systems and the deciphering of an increasing number of fungal genomes, this diverse clade of heterotrophic eukaryotic organisms has proven to be ideal for molecular work and highly serviceable as model systems to study basic processes with results that are applicable to many organisms, including humans. In Molecular and Cell Biology Methods for Fungi, experts in the field provide an up-to-date set of practical protocols covering a range of frequently used methods used to study molecular and cellular aspects of fungal biology. The included classical protocols such as transformation systems and traditional protein analysis methods, which have been widely used for many years, alongside the most advanced techniques such as genome amplification, whole genome knockout methods, and sophisticated in vivo imaging techniques will prove to be easily adaptable and useful in a wide range of species. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Molecular and Cell Biology Methods for Fungi seeks to provide cutting-edge techniques in order to aid both experienced fungal research laboratories as well as those that are interested in using fungi as hosts to study their favorite genes.
Cytology. --- Biochemistry. --- Entomology. --- Microbiology. --- Cell Biology. --- Biochemistry, general. --- Mycology. --- Eukaryotic Microbiology. --- Insects --- Zoology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Microbial biology --- Microorganisms --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition
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The book serves as an amalgamation of knowledge and principles used in the area of systems and synthetic biology, and targets inter-disciplinary research groups. The readers from diversified areas would be benefited by the valuable resources and information available in one book. Microbiome projects with efficient data handling can fuel progress in the area of microbial synthetic biology by providing a ready to use plug and play chassis. Advances in gene editing technology such as the use of tailor made synthetic transcription factors will further enhance the availability of synthetic devices to be applied in the fields of environment, agriculture and health. The different chapters of the book reviews a broad range of topics, including food microbiome in ecology, use of microbiome in personalized medicine, machine learning in biomedicine. The book also describes ways to harness and exploit the incredible amounts of genomic data. The book is not only limited to medicine but also caters to the needs of environmentalists, biochemical engineers etc. Ii will be of interest to advanced students and researchers in life sciences, computational biology, microbiology and other inter-disciplinary areas. .
Bacteriology. --- Microbiology. --- Medical microbiology. --- Systems biology. --- Biomedical engineering. --- Eukaryotic Microbiology. --- Medical Microbiology. --- Systems Biology. --- Biomedical Engineering/Biotechnology. --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Computational biology --- Bioinformatics --- Biological systems --- Molecular biology --- Microbial biology --- Biology --- Microorganisms --- Microbiology
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This book examines an important paradigm shift in biology: Plants and animals, traditionally viewed as individuals, are now considered to be complex systems and host to a plethora of microorganisms. After first presenting historical aspects of microbiota research, bacterial compositions of individual microbiomes and the critical analysis of current methods, the book discusses how microbial communities inside the human body are profoundly affected by numerous factors, such as macro- and micro-nutrients, physical exercise, antibiotics, gender and age. As described by current research, the author highlights how microbiomes contribute to the fitness of the host by providing nutrients, inhibiting pathogens, aiding in the storage of fat during pregnancy, and contributing to development and behavior. The author not only focusses on prokaryotic components in microbiomes, but also addresses single-cell eukaryotes and viruses. This follow-up to the successful book The Hologenome Concept: Human, Animal and Plant Microbiota, published in 2013, provides a contemporary overview of microbiomes. It appeals to anyone working in the life sciences and biomedicine.
Microbial ecology. --- Medical microbiology. --- Bacteriology. --- Microbiology. --- Virology. --- Microbial Ecology. --- Medical Microbiology. --- Eukaryotic Microbiology. --- Microbiology --- Microbial biology --- Biology --- Microorganisms --- Environmental microbiology --- Ecology --- Ecologia microbiana --- Ecologia dels microorganismes --- Microbiologia ambiental --- Microbiologia del medi ambient --- Ecologia --- Microbiologia --- Biofilms --- Relacions planta-microbi
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