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Semen analysis may be useful in both clinical and research settings for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility regulation and other interventions. This manual provides updated standardized evidence-based procedures and recommendations for laboratory managers scientists and technicians to follow in examining human semen in a clinical or research setting. Detailed protocols for routine optional and research tests are elaborated. The fifth edition includes new information on sperm preparation for clinical use or specialized as

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Considered the definitive text for the first course in chemistry for environmental engineers. This text has a two-fold purpose: 1) bring into focus those aspects of chemistry which are particularly valuable to environmental engineering practices, and 2) lay a groundwork of understanding in the area of specialized quantitative analysis, commonly referred to as "water and wastewater analysis."

Environmental chemistry --- Sanitary engineering --- Laboratory manuals --- Environmental chemistry. --- Sanitary engineering. --- Agrotechnology and Food Sciences. Engineering --- Laboratory manuals. --- Environmental Engineering --- Environmental Engineering. --- Environmental chemistry - Laboratory manuals --- Sanitary engineering - Laboratory manuals

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Biochemistry --- Plants --- Botanical chemistry --- Plant physiology --- Physiologie végétale --- laboratory manuals --- physiology --- Laboratory manuals --- Manuels de laboratoire --- Basic Sciences. Molecular Biology --- Laboratory manuals. --- Plant Molecular Biology --- Plant Molecular Biology. --- Physiologie végétale --- laboratory manuals. --- Botanical chemistry - Laboratory manuals --- Plant physiology - Laboratory manuals

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Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.

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