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This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.

Cell receptors. --- Lipids. --- Receptors. --- Lipidology. --- Lipides --- Lipins --- Lipoids --- Biomolecules --- Steroids --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins

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Phytochemistry is the branch of science that deals with the study of plant-derived chemicals or compounds, which are also known as phytochemicals or plant-derived secondary metabolites. Plants are known to produce phytochemicals that are essential for their growth and reproduction, as they protect them from insects, pathogens, and herbivores. Some of the major groups of plant-derived secondary metabolites are phenolics, flavonoids, terpenoids, alkaloids, tannin etc. Plant-derived phytochemicals are pharmacologically active and have the potential to cure various human diseases and disorders. Natural plant products have been known for their medicinal properties for untold years, and form the basis of several medicinal systems such as Chinese, Unani, and Ayurvedic Medicine. This book offers an essential introduction to phytochemicals and their synthetic analogues. It discusses various in silico approaches used to identify pharmacologically active phytochemicals and their biological activities, as well as in vitro and in vivo models/assays that have been utilized for the pharmacological profiling of plant-derived products to combat cancer, diabetes, cardiovascular diseases and neurological disorders. The intended audience includes upper-level undergraduate and graduate students; researchers and scientists from the pharmaceutical/food chemistry/nutrition sciences/biochemistry, and clinical biochemistry fields; and medical students. Sharing the latest findings, the book will familiarize these readers with the concepts, chemistry, and tremendous potential of phytochemistry.

Biochemistry. --- Plant physiology. --- Cell receptors. --- Plant anatomy. --- Plant Biochemistry. --- Plant Physiology. --- Receptors. --- Plant Anatomy/Development. --- Botany --- Plant structure --- Plants --- Structural botany --- Vegetable anatomy --- Anatomy --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- Physiology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Structure --- Composition --- Plant biochemistry. --- Proteins . --- Plant development. --- Development of plants --- Plant development --- Developmental biology --- Growth (Plants) --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Ontogeny