Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

Science: general issues --- Botany & plant sciences --- Aegilops sp. --- stress tolerance --- quality traits --- genome analysis --- alien introgression --- Aegilops sp. --- stress tolerance --- quality traits --- genome analysis --- alien introgression

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.

seed phosphoproteomics --- LC-ESI-MS --- purity --- transferrin --- CE-LIF --- enzyme assay --- thioglucosidase --- capillary electrophoresis --- venom composition --- immunoassay --- fluorescence --- seed proteomics --- SEC-HPLC --- desulfo-sinigrin --- metalloproteins --- seed quality traits --- seed glycoproteomics --- proteomics --- sulfatase --- on-gel detection --- non-covalent binding --- Naja ashei --- myrosinase --- SDS-PAGE --- carbon dots --- 2-D electrophoresis --- fragment --- rhIL-12 --- seed molecular breeding --- chip-based CE-LIF assay

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Metabolomics has been a useful method for various study fields. However, its application in animal science does not seem to be sufficient. Metabolomics will be useful for various studies in animal science: Animal genetics and breeding, animal physiology, animal nutrition, animal products (milk, meat, eggs, and their by-products) and their processing, livestock environment, animal biotechnology, animal behavior, and animal welfare. More application examples and protocols for animal science will promote more motivation to use metabolomics effectively in the study field. Therefore, in this Special Issue, we introduced some research and review articles for “Metabolomic Applications in Anmal Science”. The main methods used were mass spectrometry or nuclear magnetic resonance spectroscopy. Not only a non-targeted, but also a targeted, analysis of metabolites is shown. The topics include dietary and pharmacological interventions and protocols for metabolomic experiments.

Research & information: general --- Biology, life sciences --- Technology, engineering, agriculture --- albumen --- breed --- chicken --- feed --- metabolome --- yolk --- arachidonic acid --- omega-3 fatty acids --- lipidomics --- mass spectrometry --- dietary fat --- fatty acid metabolism --- pork --- meat --- skeletal muscle --- fiber type --- cooking --- beef --- Wagyu --- Holstein --- captive giraffes --- urine --- metabolomics --- 1H-NMR --- NMR --- metabotype --- transition --- ketosis --- cattle --- chemometrics --- spectral correction --- authentication --- biomarker --- feeding --- meat quality traits --- metabolite --- postmortem aging --- processing --- chickens --- heat stress --- lipid peroxidation --- orotic acid --- feed efficiency --- biomarkers --- SNPs --- GWAS --- RFI --- pigs --- pathways --- metabolic profile --- transition period --- livestock --- methyl donor --- one-carbon metabolism --- negative energy balance --- pasture legumes --- phytoestrogens --- flavonoids --- coumestans --- polyphenols --- proanthocyanidins --- metabolic profiling --- biosynthesis --- linear model --- transcriptomics --- horse --- metabolomic --- metabolism --- exercise --- saliva --- anabolic practices --- testosterone --- plasma --- CE-TOFMS --- intramuscular fat --- meat quality --- porcine --- albumen --- breed --- chicken --- feed --- metabolome --- yolk --- arachidonic acid --- omega-3 fatty acids --- lipidomics --- mass spectrometry --- dietary fat --- fatty acid metabolism --- pork --- meat --- skeletal muscle --- fiber type --- cooking --- beef --- Wagyu --- Holstein --- captive giraffes --- urine --- metabolomics --- 1H-NMR --- NMR --- metabotype --- transition --- ketosis --- cattle --- chemometrics --- spectral correction --- authentication --- biomarker --- feeding --- meat quality traits --- metabolite --- postmortem aging --- processing --- chickens --- heat stress --- lipid peroxidation --- orotic acid --- feed efficiency --- biomarkers --- SNPs --- GWAS --- RFI --- pigs --- pathways --- metabolic profile --- transition period --- livestock --- methyl donor --- one-carbon metabolism --- negative energy balance --- pasture legumes --- phytoestrogens --- flavonoids --- coumestans --- polyphenols --- proanthocyanidins --- metabolic profiling --- biosynthesis --- linear model --- transcriptomics --- horse --- metabolomic --- metabolism --- exercise --- saliva --- anabolic practices --- testosterone --- plasma --- CE-TOFMS --- intramuscular fat --- meat quality --- porcine

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Over the past ten years, metabolomics strategies have allowed the relative or absolute quantitation of metabolite levels for the study of various biological questions in plant sciences. For fruit studies, in particular, they have participated in the identification of the genes underpinning fruit development and ripening. This book proposes examples of the current use of metabolomics studies of fruit for basic research or practical applications. It includes articles about several tropical and temperate fruit species. The studies concern fruit biochemical phenotyping, fruit metabolism during development and after harvest, including primary and specialized metabolisms, or bioactive compounds involved in fruit growth and environmental responses. The analytical strategies used are based mostly on liquid or gas chromatography coupled with mass spectrometry, but also on nuclear magnetic resonance and near-infrared spectroscopy. The effect of genotype, stages of development, or fruit tissue type on metabolomic profiles and corresponding metabolism regulations are addressed for fruit metabolism studies. The interest in combining other omics with metabolomics is also exemplified.

Research & information: general --- Biology, life sciences --- Anacardium occidentale --- fast phenotyping --- NIR --- UPLC-HRMS --- chemometrics --- Capsicum frutescens L. --- non-targeted metabolomics --- secondary metabolism --- Liquid Chromatography coupled to Mass Spectrometry (LC-MS) --- mulberry --- high resolution mass spectrometry --- antioxidant activity --- in vitro gastrointestinal digestion --- α-glucosidase inhibitory activity --- cytokinin --- fruit expansion --- kiwifruit --- phytohormone --- tomato --- metabolomics --- biochemical phenotyping --- priming --- BABA --- Botrytis cinerea --- Phytophthora infestans --- Pseudomonas syringae --- fruit pigmentation --- introgression lines --- mass spectrometry --- San Marzano landrace --- Davidson’s plum --- finger lime --- native pepperberry --- antioxidant --- amino acids --- GC×GC-TOFMS --- UHPLC-QqQ-TOF-MS/MS --- bush fruit --- genetic resources --- melon --- genotype by sequencing --- elemental analysis --- metabolome --- Cucumis melo --- pineapple --- ripening --- non-climacteric --- lipophilic compounds --- lipid-related genes --- lipid metabolism --- fruit --- postharvest --- quality traits --- stress --- biomarkers --- polyphenolics --- solanaceous crops --- capsicum annuum --- pepper --- eggplant --- fruit ripening --- tissue-specificity --- flavonoid --- wine --- clones --- Vitis vinifera --- sensory analysis --- fruit metabolomics --- developmental metabolomics --- stress metabolomics --- spatial metabolomics --- central metabolism --- specialized metabolism --- nuclear magnetic resonance spectroscopy --- omics --- multi-omics integration

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Over the past ten years, metabolomics strategies have allowed the relative or absolute quantitation of metabolite levels for the study of various biological questions in plant sciences. For fruit studies, in particular, they have participated in the identification of the genes underpinning fruit development and ripening. This book proposes examples of the current use of metabolomics studies of fruit for basic research or practical applications. It includes articles about several tropical and temperate fruit species. The studies concern fruit biochemical phenotyping, fruit metabolism during development and after harvest, including primary and specialized metabolisms, or bioactive compounds involved in fruit growth and environmental responses. The analytical strategies used are based mostly on liquid or gas chromatography coupled with mass spectrometry, but also on nuclear magnetic resonance and near-infrared spectroscopy. The effect of genotype, stages of development, or fruit tissue type on metabolomic profiles and corresponding metabolism regulations are addressed for fruit metabolism studies. The interest in combining other omics with metabolomics is also exemplified.

Anacardium occidentale --- fast phenotyping --- NIR --- UPLC-HRMS --- chemometrics --- Capsicum frutescens L. --- non-targeted metabolomics --- secondary metabolism --- Liquid Chromatography coupled to Mass Spectrometry (LC-MS) --- mulberry --- high resolution mass spectrometry --- antioxidant activity --- in vitro gastrointestinal digestion --- α-glucosidase inhibitory activity --- cytokinin --- fruit expansion --- kiwifruit --- phytohormone --- tomato --- metabolomics --- biochemical phenotyping --- priming --- BABA --- Botrytis cinerea --- Phytophthora infestans --- Pseudomonas syringae --- fruit pigmentation --- introgression lines --- mass spectrometry --- San Marzano landrace --- Davidson’s plum --- finger lime --- native pepperberry --- antioxidant --- amino acids --- GC×GC-TOFMS --- UHPLC-QqQ-TOF-MS/MS --- bush fruit --- genetic resources --- melon --- genotype by sequencing --- elemental analysis --- metabolome --- Cucumis melo --- pineapple --- ripening --- non-climacteric --- lipophilic compounds --- lipid-related genes --- lipid metabolism --- fruit --- postharvest --- quality traits --- stress --- biomarkers --- polyphenolics --- solanaceous crops --- capsicum annuum --- pepper --- eggplant --- fruit ripening --- tissue-specificity --- flavonoid --- wine --- clones --- Vitis vinifera --- sensory analysis --- fruit metabolomics --- developmental metabolomics --- stress metabolomics --- spatial metabolomics --- central metabolism --- specialized metabolism --- nuclear magnetic resonance spectroscopy --- omics --- multi-omics integration