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This book is an authoritative monograph on the recent progresses in the chemistry of bioluminescence. It provides a comprehensive overview of the past and the latest developments in understanding the biochemical mechanisms of some 35 different types of luminous organisms, together with information helpful to students and researchers in an Appendix. It is the first and only book that provides chemical information on all currently known bioluminescence systems. Dr Shimomura is the leading practitioner in the field for the past half century, and is best known for his discovery of the jellyfish photoprotein aequorin and the green fluorescent protein. This book is the bible of bioluminescence, and is "a must read", not only for the students who study bioluminescence but also for those who work in various aspects relating to bioluminescence. This book will be an important source of chemical knowledge on bioluminescence for a long period of time in future. Fully revised since its publication in 2006, it now incorporates the most recent advances in the subject area. A new section on "Green Fluorescent Protein" has been added at the end of Chapter 4. It also contains an extensive reference section.

Luminescence --- Luminescent Proteins --- chemistry --- Bioluminescence. --- Chemiluminescence. --- chemistry. --- Chemoluminescence --- Animal light --- Animal luminescence --- Light production in organisms --- Photobiology --- Luminescence.

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Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.

Conjugated polymers. --- Fluorescence spectroscopy. --- Imaging systems in biology. --- Imaging systems in chemistry. --- Fluorescence spectroscopy --- Coloring Agents --- Proteins --- Luminescent Agents --- Natural Science Disciplines --- Fluorometry --- Spectrum Analysis --- Microscopy --- Laboratory Chemicals --- Luminescent Proteins --- Fluorescent Dyes --- Chemistry --- Microscopy, Fluorescence --- Spectrometry, Fluorescence --- Indicators and Reagents --- Disciplines and Occupations --- Luminescent Measurements --- Investigative Techniques --- Diagnostic Imaging --- Chemistry Techniques, Analytical --- Amino Acids, Peptides, and Proteins --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Diagnostic Techniques and Procedures --- Photometry --- Diagnosis --- Analytical Chemistry --- Physical Sciences & Mathematics --- Chemical imaging systems --- Biological imaging systems --- Fluorescence spectrometry --- Spectroscopy, Fluorescence --- Chemistry. --- Molecular biology. --- Analytical chemistry. --- Medical biochemistry. --- Biology --- Biophysics. --- Biological physics. --- Optical materials. --- Electronic materials. --- Analytical Chemistry. --- Molecular Medicine. --- Biophysics and Biological Physics. --- Medical Biochemistry. --- Optical and Electronic Materials. --- Biological Techniques. --- Technique. --- Chemical apparatus --- Organic semiconductors --- Polymers --- Conducting polymers --- Luminescence spectroscopy --- Fluorescent probes --- Analytical biochemistry. --- Medicine. --- Biochemistry. --- Cytology --- Biological and Medical Physics, Biophysics. --- Research --- Methodology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Optics --- Materials --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Analytic biochemistry --- Biochemistry --- Chemistry, Analytic --- Composition --- Health Workforce --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Biology—Technique. --- Electronic materials --- Medical biochemistry --- Pathobiochemistry --- Pathological biochemistry --- Biological physics --- Physics --- Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis

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Microscopy, Confocal --- Microscopy, Fluorescence --- Fluorescence Recovery After Photobleaching --- Luminescent Proteins --- Spectrometry, Fluorescence --- Microscopy --- Microscopie --- methods --- analysis --- Technique --- Analytical biochemistry --- Biochemistry --- Biotechnology --- Technique. --- Chemistry. --- Analytical biochemistry. --- Biotechnology. --- Biochemistry. --- Microscopy. --- Biological Microscopy. --- Analytical Chemistry. --- Biochemistry, general. --- Methods --- Spectrum Analysis --- Investigative Techniques --- Fluorometry --- Proteins --- Luminescent Measurements --- Diagnostic Imaging --- Chemistry Techniques, Analytical --- Amino Acids, Peptides, and Proteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Photometry --- Chemicals and Drugs --- Diagnostic Techniques and Procedures --- Diagnosis --- Bioengineering --- Biology --- Mechanical Engineering --- Engineering & Applied Sciences --- Health & Biological Sciences --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- Diagnostic Technics and Procedures --- Technics and Procedures, Diagnostic --- Techniques and Procedures, Diagnostic --- Photometries --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Imaging, Diagnostic --- Imaging, Medical --- Medical Imaging --- Bioluminescence Measurements --- Bioluminescent Assays --- Bioluminescent Measurements --- Chemiluminescence Measurements --- Chemiluminescent Assays --- Chemiluminescent Measurements --- Chemoluminescence Measurements --- Luminescence Measurements --- Luminescent Assays --- Luminescent Techniques --- Phosphorescence Measurements --- Phosphorescent Assays --- Phosphorescent Measurements --- Assay, Bioluminescent --- Assay, Chemiluminescent --- Assay, Luminescent --- Assay, Phosphorescent --- Assays, Bioluminescent --- Assays, Chemiluminescent --- Assays, Luminescent --- Assays, Phosphorescent --- Bioluminescence Measurement --- Bioluminescent Assay --- Bioluminescent Measurement --- Chemiluminescence Measurement --- Chemiluminescent Assay --- Chemiluminescent Measurement --- Chemoluminescence Measurement --- Luminescence Measurement --- Luminescent Assay --- Luminescent Measurement --- Luminescent Technique --- Measurement, Bioluminescence --- Measurement, Bioluminescent --- Measurement, Chemiluminescence --- Measurement, Chemiluminescent --- Measurement, Chemoluminescence --- Measurement, Luminescence --- Measurement, Luminescent --- Measurement, Phosphorescence --- Measurement, Phosphorescent --- Measurements, Bioluminescence --- Measurements, Bioluminescent --- Measurements, Chemiluminescence --- Measurements, Chemiluminescent --- Measurements, Chemoluminescence --- Measurements, Luminescence --- Measurements, Luminescent --- Measurements, Phosphorescence --- Measurements, Phosphorescent --- Phosphorescence Measurement --- Phosphorescent Assay --- Phosphorescent Measurement --- Technique, Luminescent --- Techniques, Luminescent --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Fluorometric Analysis --- Fluorimetry --- Analysis, Fluorometric --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Microscopies --- Analysis, Spectrum --- Spectrometry --- Spectroscopy --- Fluorescence Microscopy --- Immunofluorescence Microscopy --- Microscopy, Immunofluorescence --- Fluorescence Microscopies --- Immunofluorescence Microscopies --- Microscopies, Fluorescence --- Microscopies, Immunofluorescence --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Bioluminescent Proteins --- Photoproteins --- Proteins, Bioluminescent --- Proteins, Luminescent --- Fluorescence Spectrophotometry --- Fluorescence Spectroscopy --- Spectrofluorometry --- Fluorescence Spectrometry --- Spectrophotometry, Fluorescence --- Spectroscopy, Fluorescence --- Confocal Laser Scanning Microscopy --- Confocal Microscopy --- Confocal Microscopy, Scanning Laser --- Laser Microscopy --- Laser Scanning Confocal Microscopy --- Laser Scanning Microscopy --- Microscopy, Confocal, Laser Scanning --- Confocal Microscopies --- Laser Microscopies --- Laser Scanning Microscopies --- Microscopies, Confocal --- Microscopies, Laser --- Microscopies, Laser Scanning --- Microscopy, Laser --- Microscopy, Laser Scanning --- Scanning Microscopies, Laser --- Scanning Microscopy, Laser --- Microscopical technique --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Analytic biochemistry --- diagnosis --- Composition --- FRAP (Fluorescence Recovery After Photobleaching) --- Fluorescence Photobleaching Recovery --- FRAPs (Fluorescence Recovery After Photobleaching) --- Analytical chemistry. --- Life sciences. --- Life Sciences, general. --- Optics --- Chemistry --- Medical sciences --- Chemical engineering --- Genetic engineering --- Physical sciences --- Biosciences --- Sciences, Life --- Science --- Analysis, Chemical --- Analytical chemistry --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- Chemistry, Analytic --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytic chemistry --- Microscopy - Technique.