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RECEPTORS, DOPAMINE --- BIOANALYSIS --- DRUG EFFECTS --- RECEPTORS, DOPAMINE --- BIOANALYSIS --- DRUG EFFECTS
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Der Beitrag von Maria Mulisch bietet einen Überblick über die Techniken der Immunmarkierung für licht- und elektronenmikroskopische Präparate. Es werden Grundlagen der Eigenschaften, Auswahl, Reinigung und Auswahl von Antikörpern vermittelt und verschiedene Detektionssysteme (Enzyme, Fluorochrome, kolloidales Gold) und Signalverstärkungsmethoden vorgestellt und durch Tabellen ergänzt. Probleme und Fehlerquellen sind tabellarisch zusammengefasst und bieten übersichtlich Lösungsmöglichkeiten. Ausgewählte, detaillierte Anleitungen ergänzen und veranschaulichen den Text. Der Inhalt Antikörper Detektionsverfahren Direkte und indirekte Immunmarkierung Signalverstärkungsverfahren Whole mount-Immunmarkierung Preembedding-Techniken Kontrollen und Problembehandlung Die Zielgruppen Dozierende und Studierende der Biologie, Pharmazie und Medizin Die Autorin Maria Mulisch lehrt, forscht und arbeitet seit mehr als 30 Jahren im Bereich Zellbiologie mit Hilfe von Licht- und elektronenmikroskopischen Methoden. Sie hat an der Universität Heidelberg promoviert, an der Universität zu Köln habilitiert und ist jetzt wissenschaftliche Leiterin der Zentralen Mikroskopie an der CAU Kiel. .
Biology --- Biophysics. --- Biochemistry. --- Biological Techniques. --- Bioanalysis and Bioimaging. --- Technique.
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Tissue-Printing ist eine schnelle und einfache Methode, Moleküle in biologischen Proben nachzuweisen und im Gewebeverband zu lokalisieren. Maria Mulisch stellt Techniken für unterschiedliche Gewebetypen vor. Detaillierte, beispielhafte Schritt-für-Schritt-Anleitungen ermöglichen es auch Anfängern, Proteine, Nukleinsäuren und Enzymaktivität in Pflanzengewebe aufzufinden. Der Inhalt Anfertigen von Tissue-Prints Western Tissue-Print Northern Tissue-Print Coomassie-Färbung Peroxidase-Lokalisation Die Zielgruppen Dozierende und Studierende der Biologie, Pharmazie und Medizin Biologielehrer Die Autorin Maria Mulisch lehrt, forscht und arbeitet seit mehr als 30 Jahren im Bereich Zellbiologie mit Hilfe von Licht- und elektronenmikroskopischen Methoden. Sie hat an der Universität Heidelberg promoviert, an der Universität zu Köln habilitiert und ist jetzt wissenschaftliche Leiterin der Zentralen Mikroskopie an der CAU Kiel.
Biochemistry. --- Microbiology. --- Biology --- Biophysics. --- Biological Techniques. --- Bioanalysis and Bioimaging. --- Technique.
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Rolf T. Borlinghaus erläutert die Ursachen für die klassische Begrenzung der Lichtmikroskopie und beleuchtet die neuen Super-Hochauflösungstechniken. Dies ist besonders aktuell, da der Nobelpreis 2014 für Chemie für die Entwicklung von Technologien vergeben wurde, die es nun ermöglichen, mit Lichtmikroskopen feinere Details aufzulösen, als es die klassische Theorie einschränkend vorhersagt. Diese neuen Methoden stellen aber nicht das bisherige Weltbild der Optik in Frage, vielmehr nutzen sie ganz andere Phänomene, um mittels klassischer Optik Positionsbestimmungen von Molekülen durchzuführen. Das ist theoretisch beliebig genau möglich. Der Inhalt Was ist Auflösung? Das klassische Lichtmikroskop Exakte Positionsbestimmungen Mehr Details durch weniger Licht Die Zielgruppen Dozierende und Studierende der Biologie, Medizin und Philosophie Praktiker aus der Biologie, Biomedizin, Polymerforschung, Mineralogie und Nahrungsmittelindustrie sowie an Naturwissenschaft und Technik interessierte Laien Der Autor Dr. rer. nat. Rolf Theodor Borlinghaus ist promovierter Biophysiker und arbeitet seit 1990 für verschiedene Unternehmen in der Lichtmikroskopie in Deutschland.
Medicine --- Biology --- Biophysics. --- Lasers. --- Biomedical Research. --- Bioanalysis and Bioimaging. --- Laser. --- Research.
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This book presents cutting-edge research and developments in the field of medical and biological engineering, which a special emphasis on activities carried out in the Asian-Pacific region. Gathering the proceedings of the 12th Asian-Pacific Conference on Medical and Biological Engineering (APCMBE 2023), held on May 18–21, 2023, in Suzhou, China, this second volume of a two-volume set covers advances in computer-aided surgery, biomechanics and micro-nanoengineering, health informatics and health engineering, as well as computational modeling and simulation, as well as AI applications in biology and medicine. It addresses a broad audience of researchers and professionals active in biomedical engineering, biomechanics, medical biophysics, and health informatics.
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The book gives an insight into the thorough study and examination of incumbent biosystems, their present status and disruption in their integrity, causes and effects, measures to be taken for their characterization and restoration apart from advances and applications in the field of biosciences, drug design, discovery, bio-systems, biomedical and drug delivery technologies, tools in particular. The book collates information from several disciplines, such as chemistry, biology, material science, engineering, statistics, biomedicine, genetics, etc., as the subject in question is a confluence of many disciplines exhibiting numerous applications such as bioimaging, novel biological agents, synthesis, discovery testing, characterization of drugs right from selecting a suitable precursor to discovering and designing a drug following a correct synthetic route, adoption of computer simulation-based models, AI/ML-based models, application of statistical tools in analyzing and interpreting data, design, multi-functional, and operational drug delivery systems, their bio-compatibility, capacity of carrying and release of drug reproducibly etc. The book is helpful to postgraduate students, research scholars, academicians, and scientists from the pharmaceutical, biotechnology, and chemical engineering domains. The book covers a conceptual understanding of the exploration of drugs in unity with the applications desired, sound bio-system development, and carriers for drug and supplement delivery.
Biophysics. --- Imaging systems in biology. --- Biomaterials. --- Pharmacology. --- Bioanalysis and Bioimaging. --- Biological Imaging. --- Biomedical Materials.
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This book presents the fundamental and applied properties of different phosphor materials that exhibit the phenomenon of persistent luminescence (PerLum). PerLum offers a solution for the limitations of existing fluorescent probes, with new lanthanide-based materials for in vivo bio-imaging and demonstrates the practical applications of these materials. The book offers a basic understanding of PerLum, different mechanisms, models, related spectroscopic techniques, and a few applications essential for anyone interested in learning about PerLum materials. This is partly due to the fact that the property of long-lasting luminescence is closely associated with other properties and functionalities, which besides being essential for many technological applications, such as bioimaging and biosensing, involve in-depth physics and chemistry. Although the concept of luminescence has been explored for many decades, the different types of luminescence have always led to versatile breakthroughs in different fields of science and technology. Among the same is one more well-established phenomenon known as "PerLum," which is characterized by the maintenance of luminescence for periods ranging from minutes to hours in the absence of an external excitation source. Because of their remarkable properties, an enormous number of PerLum materials have been developed and are now widely used in a variety of fields, particularly bioimaging, biosensing, therapeutics, etc.
Optics. --- Nanotechnology. --- Nanobiotechnology. --- Biophysics. --- Applied Optics. --- Nanoengineering. --- Bioanalysis and Bioimaging. --- Luminescence.
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Photoacoustic imaging (also called optoacoustic imaging) is a hybrid modality based on the generation and detection of ultrasound in response to optical absorption of tissue. It combines advantages from both optical and ultrasound imaging, providing functional, molecular and microstructural information of tissue at scalable spatial resolution and depth. This technology has undergone exponential growth over the last two decades, and it is now widely viewed as one of the most exciting biomedical imaging modalities. This book introduces the technology and applications with chapters written by leading international research groups. It will be of interest to a wide range of audiences, including postgraduate students and researchers in physics and engineering as well as biomedical and clinical sciences. Chapters 8, 16, 17 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Biophysics. --- Acoustics. --- Materials --- Imaging systems. --- Nuclear medicine. --- Bioanalysis and Bioimaging. --- Imaging Techniques. --- Nuclear Medicine. --- Analysis.
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This book provides a comprehensive survey of coded optical imaging. Illustrated with 386 figures, it takes readers from the fundamental concepts and theories to the latest research and applications in this field. It can be used in graduate-level courses in optics and photonics. It can also benefit scientists and engineers in optical imaging, computer graphics, and other related disciplines. This book starts from a brief history of coded optical imaging and key operations in its data acquisition and image reconstruction. It then presents the latest progress in technological development and applications in the areas of biomedicine, materials science, industrial inspection, optical physics, imaging science, information theory, and more. Chapters describe the most representative techniques, exposing readers to key research themes, including: · Optical signal encoding · Image reconstruction techniques · Compressed sensing · Artificial intelligence · Metasurface · Structured light · Lensless imaging · Holography · Tomography · Light-field imaging · Utrafast imaging · Hyperspectral imaging · Polarization imaging · Super-resolution imaging.
Optics. --- Imaging systems in biology. --- Biophysics. --- Spectrum analysis. --- Optics and Photonics. --- Biological Imaging. --- Bioanalysis and Bioimaging. --- Spectroscopy.
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This book gathers selected, extended and revised contributions to the 18th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering, held on May 3-5, 2023, at Arts Et Métiers - Institute Of Technology, in Paris, France. They highlight cutting-edge advances in computational modelling in biomedical engineering, discusses new developments on imaging and visualization, as well as solutions for applying them in the clinical practice. All in all, this book offers a timely snapshot of the latest research and current challenges at the interface between biomedical engineering, computational biomechanics and biological imaging. It also aims at fostering future, cross-disciplinary collaborations.
Biomedical engineering. --- Biomechanics. --- Biophysics. --- Cell interaction. --- Biomechanical Analysis and Modeling. --- Bioanalysis and Bioimaging. --- Mechanobiological Cell Signaling. --- Biomechanics
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