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This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing.A theoretical introduction and practical guide for molecular biologi
RNA splicing. --- Alternative Splicing. --- RNA Precursors. --- RNA Precursors --- Alternative splicing. --- Rna precursors. --- Rna splicing. --- Messenger RNA.
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Cell Nucleus --- Nucleoside Deaminases --- RNA Precursors --- Adenosine Deaminase --- RNA Processing, Post-Transcriptional --- metabolism --- genetics
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Calcitonin --- Calcitonin Gene-Related Peptide --- RNA Precursors --- RNA Splicing --- RNA, Messenger --- Transcription, Genetic --- genetics
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Biological techniques --- Enzymology --- Molecular biology --- Ribonucleic acid --- Metabolism --- ARN --- Messenger rna --- Ribonucleoproteins --- Rna precursors --- Rna splicing --- Rna, ribosomal --- Rna, transfer --- Amino acid sequence --- Nucleic acid conformation --- Rna
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