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This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation. The book consists of three sections:   Part I, entitled Lipid peroxidation and small molecule adducts focusses on the biochemical events that are involved in lipid peroxidation and to the formation of small molecules like singlet molecular oxygen. This part of the book also introduces the hexanoyl-lysine adduct. Part II, entitled Pathophysiological consequences covers a range of damaging physiological consequences of lipid peroxidation, ranging from atherosclerosis to neurodegenerative disorders. Finally, Part III, entitled Applications for diagnosis and development of functional food looks into potential diagnostic uses of lipid peroxidation, the possible beneficial effects that can be achieved and new assays in food safely that have been developed.

Lipids --- Lipids. --- Peroxidation. --- Lipides --- Lipins --- Lipoids --- Medicine. --- Cancer research. --- Food --- Posttranslational modification. --- Biomedicine. --- Cancer Research. --- Posttranslational Modification. --- Food Science. --- Biotechnology. --- Biomolecules --- Steroids --- Oncology. --- Post-translational modification . --- Food science. --- Science --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Tumors --- Synthesis --- Food—Biotechnology. --- Cancer research

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In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.

Lipoproteins --- Post-translational modification --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Lipids --- Proteins --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Synthesis

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Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.

Biochemistry. --- Post-translational modification . --- Biochemistry, general. --- Posttranslational Modification. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Synthesis --- Composition

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The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.

Biochemistry. --- Gene expression. --- Post-translational modification . --- Protein Science. --- Gene Expression. --- Posttranslational Modification. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Genes --- Genetic regulation --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Synthesis --- Expression --- Composition

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Protein Modificomics: From Modifications to Clinical Perspectives comprehensively deals with all of the most recent aspects of post-translational modification (PTM) of proteins, including discussions on diseases involving PTMs, such as Alzheimer's, Huntington's, X-linked spinal muscular atrophy-2, aneurysmal bone cyst, angelman syndrome and OFC10. The book also discusses the role PTMs play in plant physiology and the production of medicinally important primary and secondary metabolites. The understanding of PTMs in plants helps us enhance the production of these metabolites without greatly altering the genome, providing robust eukaryotic systems for the production and isolation of desired products without considerable downstream and isolation processes. Provides thorough insights into the posttranslational modifications (PTMs) of proteins in both the plant and animal kingdom. Presents diagrammatic representations of various protein modification and estimation mechanisms in four-colors. Includes coverage of diseases involving posttranslational modifications.--

Proteomics. --- Proteins. --- Protein Processing, Post-Translational. --- Amino Acid Modification, Posttranslational --- Post-Translational Amino Acid Modification --- Post-Translational Modifications --- Post-Translational Protein Processing --- Posttranslational Amino Acid Modification --- Posttranslational Modifications --- Posttranslational Protein Processing --- Protein Processing, Post Translational --- Protein Processing, Posttranslational --- Amino Acid Modification, Post-Translational --- Post-Translational Protein Modification --- Protein Modification, Post-Translational --- Amino Acid Modification, Post Translational --- Modification, Post-Translational --- Modification, Post-Translational Protein --- Modification, Posttranslational --- Modifications, Post-Translational --- Modifications, Post-Translational Protein --- Modifications, Posttranslational --- Post Translational Amino Acid Modification --- Post Translational Modifications --- Post Translational Protein Modification --- Post Translational Protein Processing --- Post-Translational Modification --- Post-Translational Protein Modifications --- Posttranslational Modification --- Processing, Post-Translational Protein --- Processing, Posttranslational Protein --- Protein Modification, Post Translational --- Protein Modifications, Post-Translational --- Glycosylation --- Histone Code --- Metabolic Detoxication, Phase II --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biology --- Proteins --- Post Translational Modification