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Genetic Engineering --- Plasmids --- Genetics, Microbial --- methods --- 579.252.5 --- 57.083 --- 57.083 Microbiological, virological, immunological methods and techniques --- Microbiological, virological, immunological methods and techniques --- 579.252.5 Extrachromosomal hereditary determinants. Episomes. Plasmide. --- Extrachromosomal hereditary determinants. Episomes. Plasmide. --- Biological techniques --- Monera --- methodology --- Microbiology --- plasmids --- Biotechnology --- ADN --- DNA --- Electrophoresis --- Microscopy --- genetic transformation --- genes --- Transcription --- transcription --- genetic engineering --- Addresses, essays, lectures --- DNA. --- transcription. --- Molecular cloning. --- Genetic Engineering - methods --- Base sequence --- Nuclease, restriction endodeoxyribo --- -Plasmids --- Vecteur de clonage --- Translation --- Hybridation de dna

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Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. ... The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.

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Molecular biology --- Recombinant DNA --- Genetic engineering --- Experiments --- Laboratory manuals --- Plasmide --- plasmids --- ADN --- DNA --- Génie génétique --- genetic engineering --- Transfert de gène --- Gene transfer --- Cloning, Molecular. --- DNA, Recombinant. --- Genetic Engineering. --- -Recombinant DNA --- -Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- DNA Transposable Elements --- Genetic Engineering --- Genetic Vectors --- Genomic Library --- Gene Library --- Molecular Cloning --- Cosmids --- Cloning, Organism --- rDNA --- Genetic vectors --- -Experiments --- Designed genetic change --- DNA. --- Recombinant DNA - Experiments --- Recombinant DNA - Laboratory manuals --- Genetic engineering - Experiments --- Exercice