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This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.

Immunology. --- Immunobiology --- Life sciences --- Serology --- Immunologia --- Bioassaigs --- Manuals de laboratori --- Tècniques de laboratori --- Assaigs biològics --- Proves biològiques --- Química analítica qualitativa --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari

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This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.

Toxoplasma gondii --- Manuals de laboratori --- Tècniques de laboratori --- Toxoplasma --- Toxoplasmosi --- Immunology. --- Immunobiology --- Life sciences --- Serology --- Immunologia --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari

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Neurology. --- Medicine --- Nervous system --- Neuropsychiatry --- Diseases --- Lesions medul·lars --- Neurologia --- Medicina --- Electrofisiologia --- Inhibició --- Neurocirurgia --- Neurooftalmologia --- Neuroimmunologia --- Neurologia pediàtrica --- Neurologia geriàtrica --- Neurologia veterinària --- Psicofisiologia --- Psicopatologia --- Urgències en neurologia --- Neuròlegs --- Lesions de medul·la espinal --- Medul·la espinal

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This book discusses novel concepts and discoveries concerning the regulation of innate immunity by autophagy and autophagy-related proteins. In the past decade, there have been major advances in our understanding of the molecular mechanisms of autophagy and its physiological functions. This book highlights emerging studies on the underlying mechanisms of autophagy regulation of innate immunity, including inflammation, antiviral immunity and anti-bacterial responses and the signaling pathways that prompt or inhibit the initiation and progression of related diseases. It also offers new ideas and strategies for future drugs based on manipulating autophagy, especially selective autophagy mediated by cargo receptors. Providing a comprehensive overview of the autophagy regulation of innate immunity, it is a valuable resource for graduate students and researchers in the fields of immunology, cell biology and translational medicine.

Immunology. --- Cell biology. --- Molecular biology. --- Cell Biology. --- Molecular Medicine. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Immunobiology --- Life sciences --- Serology --- Immunologia --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari

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This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.

Immunologia --- Metabolisme --- Immunology. --- Chemistry. --- Physiology. --- Animal physiology --- Animals --- Biology --- Anatomy --- Physical sciences --- Immunobiology --- Life sciences --- Serology --- Physiology --- Anabolisme --- Catabolisme --- Biologia --- Bioquímica --- Cicle de Krebs --- Gliconeogènesi --- Metabolisme basal --- Metabolisme cel·lular --- Metabolisme de les plantes --- Metabolisme dels lípids --- Metabolisme energètic --- Metabolisme microbià --- Metabolisme mineral --- Metabolisme secundari --- Urea --- Biosíntesi --- Nutrició --- Respiració --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari