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Nerve growth factor. --- Nervous system --- NGF (Nerve growth factor) --- Growth factors --- Nerve tissue proteins --- Pathophysiology.

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Neurosciences. --- Nerve growth factor. --- NGF (Nerve growth factor) --- Growth factors --- Nerve tissue proteins --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system

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Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system.

Nerve growth factor --- Nerve tissue proteins. --- Nerve proteins --- Neural proteins --- Neuroproteins --- Proteins --- NGF (Nerve growth factor) --- Growth factors --- Nerve tissue proteins --- Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

Science: general issues --- Neurosciences --- neurotrophic factors --- BDNF (brain-derived neurotrophic factor) --- NGF (nerve growth factor) --- CNS—central nervous system --- neuronal plasticity --- neurotrophic factors --- BDNF (brain-derived neurotrophic factor) --- NGF (nerve growth factor) --- CNS—central nervous system --- neuronal plasticity

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Facteurs de croissance nerveux --- Nerfs--Facteurs de croissance --- Nerve growth factor --- Zenuwen--Groeifactoren --- Fibroblast Growth Factors. --- Nerve Growth Factors --- Physiology --- Physiology.