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RNA interference (RNAi) is a biological process in which small nucleotide sequences destroy specific mRNA molecules and, therefore, interrupt genetic expression. RNAi has rapidly developed into a widely used technique in biotechnology, experimental biology, genomics and medicine. Frontiers in RNAi is a collection of research articles covering the applications of RNAi principles and technologies and has been compiled by members of the RNAi Global Initiative, a collection of academic and industrial groups with extensive experience in genome-wide RNAi screening. Chapters included in the first volume touch on some of the recent advances in RNAi technology (i.e., applications to 3D cell culture, drug repurposing, and bioproduction) and provide guidance on how to address the continued challenges associated with off-target effects, database structuring, and pooled shRNA screens.
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Messenger RNA. --- Messenger RNA --- Genetic regulation. --- Metabolism.
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Messenger rna. --- Rna.
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Messenger RNA --- Research --- Methodology.
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This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deadenylation: Methods and Protocols aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression.
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Cytoplasm. --- Messenger RNA --- Metabolism.
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This report by the WHO Science Council examines the potential benefits and limitations of mRNA technology for vaccine research and development targeting infectious diseases and virus-induced cancers. It highlights the success of mRNA vaccines in the COVID-19 pandemic and explores their applicability for other global health challenges. The report discusses scientific and technological advantages, issues of equitable access, and the need for further research to maximize the technology's impact. Aimed at researchers, policymakers, and healthcare professionals, it provides guidance for advancing mRNA vaccine technology within global health ecosystems.
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TOC:http://www.loc.gov/catdir/toc/wiley022/96039030.html
Genetic regulation. --- Messenger RNA. --- Messenger RNA --- Genetic regulation
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