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This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
Toxicology. --- Pharmacology/Toxicology. --- Chemicals --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Toxicology --- Agents teratògens. --- Embriologia --- Manuals de laboratori --- Tècniques de laboratori --- Desenvolupament embriològic --- Desenvolupament embrional --- Embriogènesi --- Embriogènia --- Embriologia animal --- Biologia --- Evolució --- Morfologia animal --- Edat gestacional --- Epigènesi --- Cèl·lules germinals --- Cordó umbilical --- Diferenciació sexual --- Fetus --- Gametogènesi --- Genètica del desenvolupament --- Miogènesi --- Neurobiologia del desenvolupament --- Òvuls --- Placenta --- Teratologia --- Biologia del desenvolupament --- Cèl·lules --- Protoplasma --- Reproducció --- Factors teratògens --- Medicaments teratògens --- Teratògens
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Embriologia --- Desenvolupament embriològic --- Desenvolupament embrional --- Embriogènesi --- Embriogènia --- Embriologia animal --- Biologia --- Evolució --- Morfologia animal --- Edat gestacional --- Epigènesi --- Cèl·lules germinals --- Cordó umbilical --- Diferenciació sexual --- Fetus --- Gametogènesi --- Genètica del desenvolupament --- Miogènesi --- Neurobiologia del desenvolupament --- Òvuls --- Placenta --- Teratologia --- Biologia del desenvolupament --- Cèl·lules --- Protoplasma --- Reproducció --- Placenta. --- Mammals --- Reproduction. --- Cotyledon (Anatomy) --- Embryology --- Uterus, Pregnant
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Stem cells. --- Veterinary embryology. --- Embryology --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Cèl·lules mare embrionàries --- Embriologia --- Desenvolupament embriològic --- Desenvolupament embrional --- Embriogènesi --- Embriogènia --- Embriologia animal --- Biologia --- Evolució --- Morfologia animal --- Edat gestacional --- Epigènesi --- Cèl·lules germinals --- Cordó umbilical --- Diferenciació sexual --- Fetus --- Gametogènesi --- Genètica del desenvolupament --- Miogènesi --- Neurobiologia del desenvolupament --- Òvuls --- Placenta --- Teratologia --- Biologia del desenvolupament --- Cèl·lules --- Protoplasma --- Reproducció --- Cèl·lules mare
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This atlas provides ultrastructural morphological images of human spermatozoa. Sperm morphology plays an essential role in sperm-oocyte interactions and early embryonic development, and human sperm ultrastructural morphology offers a valuable reference tool for assessing certain etiologies of male infertility and reproductive failure. However, the ultrastructural morphology of human sperm has not been systematically evaluated or thoroughly described in the literature. Using 470 original and unpublished images, the book shows various ultrastructural morphological phenotypes; defects of the sperm head, neck, middle piece, principal piece, and terminal piece; as well as artifacts of sperm ultrastructural morphology and phenomena related to inadequate preparation, demonstrating several sperm phenotypes and surface structural appearances for the first time. As such, it helps researchers and practitioners in andrology, reproductive medicine, and reproductive pathology gain a better understanding of human sperm ultrastructural morphology.
Human physiology. --- Andrology. --- Microscopy. --- Human Physiology. --- Biological Microscopy. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Generative organs, Male --- Human reproduction --- Men --- Human biology --- Medical sciences --- Physiology --- Human body --- Endocrine aspects --- Diseases --- Espermatozoides --- Semen --- Morfologia (Biologia) --- Estructura biològica --- Estructura en biología --- Forma biològica --- Morfologia comparada --- Anatomia comparada --- Homologia (Biologia) --- Malformacions --- Morfologia animal --- Morfologia vegetal --- Morfogènesi --- Humors corporals --- Espermatozous --- Gàmetes masculins --- Cèl·lules germinals --- Espermatogènesi --- Testicle
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Spermatogenesis. --- Formation of spermatozoa --- Spermatozoa --- Gametogenesis --- Formation --- Espermatogènesi --- Testicle --- Biologia molecular --- Manuals de laboratori --- Tècniques de laboratori --- Biofísica molecular --- Bioquímica molecular --- Biofísica --- Bioquímica --- Histoquímica --- Biologia molecular vegetal --- Codi genètic --- Diagnòstic molecular --- Endocrinologia molecular --- Evolució molecular --- Farmacologia molecular --- Genètica molecular --- Glicòmica --- Metabolòmica --- Microbiologia molecular --- Neurobiologia molecular --- Patologia molecular --- Proteòmica --- Reconeixement molecular --- Biomolècules --- Testicles --- Aparell genital masculí --- Espermatozoides --- Malalties del testicle --- Formació d'espermatozoides --- Gametogènesi --- Espermatogènesi en els animals --- Cèl·lules germinals
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This new textbook on bacterial physiology is aimed at senior level students pursuing a one-semester course in the biology or microbiology curriculum. The text takes a balanced view of prokaryotic physiology, discussing both bioenergetics and bacterial metabolism in a way that establishes general principles and concepts and emphasizes throughout the information gained from model systems. The book also covers some experimental design issues, giving students an appreciation of the practical aspects and consequences of bacterial metabolism. It also stimulates students’ interests in future developments in the field by including discussions by five world-famous bacterial physiologists about future developments in the field.
Prokaryotes. --- Monera --- Procaryotae --- Procaryotes --- Prokaryonta --- Prokaryotae --- Prokaryotic protists --- Microorganisms --- Bacteriology. --- Microbiology. --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Microbial biology --- Microbiology --- Composition --- Cell biology. --- Cèl·lules --- Fisiologia cel·lular --- Fisiologia --- Motilitat cel·lular --- Biologia --- Reproducció --- Astròcits --- Blastoderma --- Cèl·lules eucariotes --- Cèl·lules canceroses --- Cèl·lules epitelials --- Cèl·lules germinals --- Cèl·lules hepàtiques --- Cèl·lules i teixits vegetals --- Cèl·lules mare --- Cèl·lules musculars --- Cèl·lules sanguínies --- Citoplasma --- Cromatina --- Fecundació --- Fibroblasts --- Histologia --- Irritabilitat --- Mort cel·lular --- Neurones --- Neurones motores --- Patologia cel·lular --- Protoplasts --- Protozous --- Ou (Biologia) --- Ultraestructura (Biologia) --- Citologia --- Clonatge --- Embriologia --- Protoplasma --- Regeneració (Biologia)
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This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Immune system. --- Immunotherapy. --- Therapeutic immunology --- Clinical immunology --- Therapeutics --- Immunological system --- Anatomy --- Immunology --- Immunoteràpia --- Sistema immunitari --- Cèl·lules --- Biologia --- Fisiologia --- Reproducció --- Astròcits --- Blastoderma --- Cèl·lules eucariotes --- Cèl·lules canceroses --- Cèl·lules epitelials --- Cèl·lules germinals --- Cèl·lules hepàtiques --- Cèl·lules i teixits vegetals --- Cèl·lules mare --- Cèl·lules musculars --- Cèl·lules sanguínies --- Citoplasma --- Cromatina --- Fecundació --- Fibroblasts --- Histologia --- Irritabilitat --- Mort cel·lular --- Neurones --- Neurones motores --- Patologia cel·lular --- Protoplasts --- Protozous --- Ou (Biologia) --- Ultraestructura (Biologia) --- Citologia --- Clonatge --- Embriologia --- Protoplasma --- Regeneració (Biologia) --- Sistema immunològic --- Anatomia --- Immunologia --- Cèl·lules immunocompetents --- Medul·la òssia --- Sistema limfàtic --- Terapèutica immunològica --- Immunologia clínica --- Terapèutica --- Adjuvants immunològics --- Seroteràpia --- Timosina --- Immunology. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Immunobiology --- Life sciences --- Serology
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This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials of a cell lineage of increasing interest and therapeutic significance. .
Cèl·lules --- Sistema cardiovascular --- Aparell cardiovascular --- Aparell circulatori --- Sistema circulatori --- Sistema vascular --- Artèries --- Cor --- Sistema vasomotor --- Vasos sanguinis --- Circulació sanguínia --- Biologia --- Fisiologia --- Reproducció --- Astròcits --- Blastoderma --- Cèl·lules eucariotes --- Cèl·lules canceroses --- Cèl·lules epitelials --- Cèl·lules germinals --- Cèl·lules hepàtiques --- Cèl·lules i teixits vegetals --- Cèl·lules mare --- Cèl·lules musculars --- Cèl·lules sanguínies --- Citoplasma --- Cromatina --- Fecundació --- Fibroblasts --- Histologia --- Irritabilitat --- Mort cel·lular --- Neurones --- Neurones motores --- Patologia cel·lular --- Protoplasts --- Protozous --- Ou (Biologia) --- Ultraestructura (Biologia) --- Citologia --- Clonatge --- Embriologia --- Protoplasma --- Regeneració (Biologia) --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Biologia cel·lular --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules animals
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Histology. Cytology --- Translation science --- vertalen --- cytologie --- stamceltransplantatie --- histologie --- Medicine --- Biology --- Research. --- Biological research --- Biomedical research --- Health Workforce --- Cèl·lules --- Regeneració (Biologia) --- Medicina regenerativa --- Medicina --- Enginyeria de teixits --- Regeneració biològica --- Regeneració d'òrgans --- Biologia --- Creixement --- Regeneració del sistema nerviós --- Cicatrització --- Fisiologia --- Reproducció --- Astròcits --- Blastoderma --- Cèl·lules eucariotes --- Cèl·lules canceroses --- Cèl·lules epitelials --- Cèl·lules germinals --- Cèl·lules hepàtiques --- Cèl·lules i teixits vegetals --- Cèl·lules mare --- Cèl·lules musculars --- Cèl·lules sanguínies --- Citoplasma --- Cromatina --- Fecundació --- Fibroblasts --- Histologia --- Irritabilitat --- Mort cel·lular --- Neurones --- Neurones motores --- Patologia cel·lular --- Protoplasts --- Protozous --- Ou (Biologia) --- Ultraestructura (Biologia) --- Citologia --- Clonatge --- Embriologia --- Protoplasma
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This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. .
Cèl·lules germinals --- Malalties del testicle --- Càncer --- Malalties dels testicles --- Malalties de l'aparell genital masculí --- Malalties de les glàndules endocrines --- Criptorquídia --- Testicle --- Cèl·lules germinals primordials --- Cèl·lules reproductores --- Cèl·lules reproductives --- Cèl·lules sexuals --- Cèl·lules sexuals primordials --- Cèl·lules --- Embriologia --- Recursos genètics --- Herència (Biologia) --- Espermatozoides --- Gametogènesi --- Espermatogènesi --- Oogènesi --- Carcinoma --- Carcinosi --- Tumors --- Càncer d'ossos --- Càncer de cap --- Càncer de coll --- Càncer de bufeta --- Càncer de fetge --- Càncer de laringe --- Càncer de mama --- Càncer de pàncrees --- Càncer de pell --- Càncer de pròstata --- Càncer de pulmó --- Càncer de ronyó --- Càncer de tiroide --- Càncer d'ovari --- Càncer gastrointestinal --- Càncer ginecològic --- Càncer en els adolescents --- Càncer en els infants --- Medicaments antineoplàstics --- Melanoma --- Leucèmia --- Sarcoma --- Carcinògens --- Cèl·lules canceroses --- Cirurgia oncològica --- Infermeria oncològica --- Malalts de càncer --- Metàstasi --- Oncologia --- Developmental biology. --- Cancer. --- Cytology. --- Developmental Biology and Stem Cells. --- Cancer Biology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cancers --- Malignancy (Cancer) --- Malignant tumors --- Development (Biology) --- Growth --- Ontogeny --- Càncer genitourinari --- Citologia --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules animals --- Ultraestructura (Biologia) --- Càncer de l'aparell genitourinari --- Patologia cel·lular
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