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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptorsContains chapters

Cell receptors -- Research -- Methodology. --- Cellular signal transduction -- Research -- Methodology. --- G proteins -- Receptors. --- Signal transduction -- Physiology -- Laboratory manuals. --- Metabolism --- Biological Transport --- Receptors, Cell Surface --- Biochemical Processes --- Membrane Proteins --- Biochemical Phenomena --- Chemical Processes --- Metabolic Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Receptors, G-Protein-Coupled --- Protein Transport --- Protein Binding --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- G proteins --- Receptors. --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Enzymology.

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Continuing progress has been made in understanding the brain at the molecular, anatomic, and physiological levels in the years following the "Decade of the Brain," with the results providing insight into the underlying basis of many neurological disease processes. In Neuroscience in Medicine, Third Edition, a distinguished panel of basic and clinical investigators, noted for their teaching excellence, provide thoroughly updated and revised chapters to reflect these remarkable advances. Designed specifically for medical students and allied health professionals, this up-to-date edition alternates scientific and clinical chapters that explain the basic science underlying neurological processes and then relate that science to the understanding of neurological disorders and their treatment. These popular and now expanded "clinical correlations" cover, in detail, disorders of the spinal cord, neuronal migration, the autonomic nervous system, the limbic system, ocular motility, and the basal ganglia, as well as demyelinating disorders, stroke, dementia and abnormalities of cognition, congenital chromosomal and genetic abnormalities, Parkinson's disease, nerve trauma, peripheral neuropathy, aphasias, sleep disorders and myasthenia gravis. In addition to concise summaries of the most recent biochemical, physiological, anatomical, and behavioral advances, the chapters summarize current findings on neuronal gene expression and protein synthesis at the molecular level. Authoritative and comprehensive, Neuroscience in Medicine, Third Edition provides a fully up-to-date and readily accessible guide to brain functions at the cellular and molecular level, as well as clearly demonstrating their emerging diagnostic and therapeutic importance.

Medicine. --- Human physiology. --- Neurosciences. --- Neurology. --- Neurobiology. --- Biomedicine. --- Human Physiology. --- Neurosciences --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Medicine --- Nervous system --- Neuropsychiatry --- Neural sciences --- Neurological sciences --- Neuroscience --- Physiology --- Human body --- Diseases --- Neurophysiology. --- Pathophysiology. --- Neurobiology --- Neurology .

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Gene Probes

Biological techniques --- Neuropathology --- Molecular biology --- Neurobiology --- DNA probes --- Gene expression --- Neurobiologie --- Biologie moléculaire --- Sondes ADN --- Expression génique --- Technique --- Biologie moléculaire --- Expression génique --- Mammals --- Gene expression. --- Hybridization. --- Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Hybridisation --- Hybridism --- Breeding --- Heredity --- Mendel's law --- Genes --- Genetic regulation --- Nervous system. --- Expression

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The developing new field of proteomics holds significant promise for a deeper understanding of complicated biological systems, as well as for novel approaches to the cure of disease. In the Handbook of Proteomic Methods, P. Michael Conn has assembled a panel of well-recognized experts to describe a wide range of powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. These readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, post-translational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology. Each fully tested protocol is described in step-by-step detail, complete with tricks of the trade and hints on avoiding pitfalls. The detail of description exceeds that usually found in primary publications. Wide-ranging and practical, the Handbook of Proteomic Methods offers scientists entering, or already active in, the field a fully referenced compendium of easy-to-use proteomic methods-each reflecting the power and promise of proteomics in illuminating complex biological systems and in promoting the development of novel drugs for the amelioration of disease.

Analytical biochemistry --- Proteomics --- Biochemistry --- Genomics --- Computational Biology --- Biological Science Disciplines --- Genetics --- Chemistry --- Biology --- Natural Science Disciplines --- Disciplines and Occupations --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition --- Molecular biology --- Proteins

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Eiwit [Groene fluorescente ] --- GFP --- Green fluorescent protein --- Groene fluorescente eiwit --- Protéine fluorescente verte --- Protéine verte fluorescente --- FLUORESCENT DYES --- PROTEINS --- BIOLOGICAL STUDIES --- Enzymologie