TY - BOOK ID - 943807 TI - Proteome research : two-dimensional gel electrophoresis and identification methods PY - 2000 SN - 3540657924 3540656898 3642571050 9783540656890 PB - New York, NY ; Berlin : Springer-Verlag, DB - UniCat KW - Analytical biochemistry KW - Fysicochemical separation methods KW - Proteins KW - Gel electrophoresis KW - Protéines KW - Electrophorèse en gel KW - Analysis KW - Analyse KW - Électrophorèse KW - Electrophoresis KW - Identification KW - identification KW - 577.112 KW - 577.218 KW - 543.545 KW - 57.088 KW - -#ABIB:atte KW - Proteids KW - Biomolecules KW - Polypeptides KW - Proteomics KW - Molecular mechanisms of control : gene expression. Molecular embryology KW - Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography KW - Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - Gel electrophoresis. KW - Analysis. KW - Life sciences. KW - Cancer research. KW - Human genetics. KW - Molecular biology. KW - Biochemistry. KW - Proteomics. KW - Life Sciences. KW - Biochemistry, general. KW - Human Genetics. KW - Molecular Medicine. KW - Cancer Research. KW - 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography KW - 577.218 Molecular mechanisms of control : gene expression. Molecular embryology KW - 577.112 Proteins KW - Protéines KW - Electrophorèse en gel KW - #ABIB:atte KW - identification. KW - Cancer research KW - Molecular biochemistry KW - Molecular biophysics KW - Biochemistry KW - Biophysics KW - Systems biology KW - Genetics KW - Heredity, Human KW - Human biology KW - Physical anthropology KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Molecular biology KW - Composition KW - Proteins - Analysis KW - Proteome UR - https://www.unicat.be/uniCat?func=search&query=sysid:943807 AB - Proteomics, a branch of functional genomics, can be defined as the large-scale analysis of proteins. In all these large-scale analysis processes there are three phases: (1) separation of the molecules to be analyzed; (2) characterization of the separated molecules; and (3) managing, handling and analysis of the data. This book is centered on the first two phases of proteomics, i.e. separation by two-dimensional electrophoresis and microcharacterization of the separated proteins. This choice is due to the fact that the data production phase is the major bottleneck of proteomics, while it is not the case in standard genomics. Emphasis has been placed on the rationale of each technique, so that the reader will be able to keep pace with the rapid evolution of the techniques. However, up-to-date protocols are also given to help the beginner in the field. ER -