TY - BOOK ID - 93651658 TI - Biophysics and the Challenges of Emerging Threats AU - Puglisi, Joseph D AU - SpringerLink (Online service) PY - 2009 SN - 9789048123681 PB - Dordrecht Springer Netherlands DB - UniCat KW - Solid state physics KW - Chemical and physical crystallography KW - Chemistry KW - General biophysics KW - Biotechnology KW - Computer. Automation KW - kristallografie KW - biofysica KW - chemie KW - informatica KW - spectroscopie KW - biotechnologie KW - fysica UR - https://www.unicat.be/uniCat?func=search&query=sysid:93651658 AB - Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1-3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4-6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11-13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16-18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels. ER -