TY - BOOK ID - 9066495 TI - Neuropeptide Y Protocols PY - 2000 SN - 0896036626 9786610821631 1280821639 159259042X PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Electronic books. -- local. KW - Genetics -- Laboratory manuals. KW - Neuropeptide Y -- Laboratory manuals. KW - Neuropeptide Y KW - Neuropeptides KW - Peptides KW - Nerve Tissue Proteins KW - Amino Acids, Peptides, and Proteins KW - Proteins KW - Chemicals and Drugs KW - Animal Biochemistry KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Genetics KW - Neuropeptide tyrosine KW - NPY (Neuropeptide) KW - Biology KW - Embryology KW - Mendel's law KW - Adaptation (Biology) KW - Breeding KW - Chromosomes KW - Heredity KW - Mutation (Biology) KW - Variation (Biology) KW - Neurosciences. KW - Neural sciences KW - Neurological sciences KW - Neuroscience KW - Medical sciences KW - Nervous system UR - https://www.unicat.be/uniCat?func=search&query=sysid:9066495 AB - It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning. Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders. ER -