TY - BOOK ID - 8790216 TI - Natural killer cell protocols : cellular and molecular methods AU - Campbell, Kerry S. AU - Colonna, Marco. PY - 2000 SN - 0896036839 0585400652 9786610820368 1280820365 1592590446 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Electronic books. -- local. KW - Killer cells -- Immunology. KW - Killer cells -- Laboratory manuals. KW - Killer cells KW - Killer Cells, Natural KW - Cytotoxicity Tests, Immunologic KW - Methods KW - Lymphocytes KW - Investigative Techniques KW - Toxicity Tests KW - Immunologic Tests KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Laboratory Techniques and Procedures KW - Immunologic Techniques KW - Leukocytes, Mononuclear KW - Leukocytes KW - Diagnosis KW - Immune System KW - Blood Cells KW - Cells KW - Hemic and Immune Systems KW - Blood KW - Anatomy KW - Microbiology & Immunology KW - Biology KW - Health & Biological Sciences KW - immunology. KW - methods. KW - Immunology. KW - K cells KW - Natural killer cells KW - NK cells KW - Immunocompetent cells KW - Cell-mediated cytotoxicity KW - Immunobiology KW - Life sciences KW - Serology UR - https://www.unicat.be/uniCat?func=search&query=sysid:8790216 AB - In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells. ER -