TY - BOOK ID - 8285739 TI - Sperm Acrosome Biogenesis and Function During Fertilization PY - 2016 SN - 3319305654 3319305670 PB - Cham : Springer International Publishing : Imprint: Springer, DB - UniCat KW - Medicine. KW - Human physiology. KW - Molecular biology. KW - Reproductive medicine. KW - Urology. KW - Biomedicine. KW - Human Physiology. KW - Molecular Medicine. KW - Reproductive Medicine. KW - Spermatozoa. KW - Fertilization (Biology) KW - Acrosome reaction. KW - Acrosomal reaction KW - Reaction, Acrosome KW - Amphimixis KW - Male gametes KW - Sperm KW - Medicine KW - Genitourinary organs KW - Human reproduction KW - Human reproductive health KW - Human reproductive medicine KW - Reproductive medicine KW - Health KW - Molecular biochemistry KW - Molecular biophysics KW - Biochemistry KW - Biophysics KW - Biomolecules KW - Systems biology KW - Human biology KW - Medical sciences KW - Physiology KW - Human body KW - Clinical sciences KW - Medical profession KW - Life sciences KW - Pathology KW - Physicians KW - Diseases KW - Health aspects KW - Chemical reactions KW - Sperm-ovum interactions KW - Spermatozoa KW - Biology KW - Cells KW - Embryology KW - Reproduction KW - Gametes KW - Semen KW - Health Workforce KW - Reproductive health. KW - Biomedical Research. KW - Research. KW - Biological research KW - Biomedical research UR - https://www.unicat.be/uniCat?func=search&query=sysid:8285739 AB - Over the last decades, acrosomal exocytosis (also called the “acrosome reaction”) has been recognized as playing an essential role in fertilization. Secretion of this granule is an absolute requirement for physiological fertilization. In recent years, the study of mammalian acrosomal exocytosis has yielded some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. From a molecular point of view, acrosome exocytosis is a synchronized and tightly regulated process mediated by molecular mechanisms that are homologous to those reported in neuroendocrinal cell secretions. The authors provide a broader perspective, focusing on a limited number of important topics that are essential for understanding the molecular mechanisms governing this step in the fertilization process. They also discuss molecular aspects such as the signaling pathways leading to exocytosis, including the participation of ion channels, lipids, the fusion machinery proteins and the actin cytoskeleton as well as cellular aspects such as the site of acrosomal exocytosis and the use of gene-manipulated animals to study this process. . ER -