TY - BOOK ID - 7542662 TI - Guidelines for molecular analysis in archive tissues PY - 2011 SN - 3642178898 3642178901 PB - Berlin ; New York : Springer, DB - UniCat KW - Biochemistry. KW - Biomedical engineering -- Moral and ethical aspects. KW - Human body -- Moral and ethical aspects. KW - Pathology. KW - Pathology, Molecular KW - Clinical Laboratory Techniques KW - Genetic Techniques KW - Complex Mixtures KW - Tissue Preservation KW - Chemicals and Drugs KW - Investigative Techniques KW - Histocytological Preparation Techniques KW - Histological Techniques KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Cytological Techniques KW - Sequence Analysis KW - Molecular Diagnostic Techniques KW - Tissue Extracts KW - Tissue Fixation KW - Medicine KW - Health & Biological Sciences KW - Pathology KW - Pharmacy, Therapeutics, & Pharmacology KW - Preservation of organs, tissues, etc. KW - Tissue banks. KW - Banks, Organ KW - Banks, Tissue KW - Banks, Transplant KW - Organ banks KW - Transplant banks KW - Organ preservation (Anatomy) KW - Organs (Anatomy) KW - Preservation KW - Medicine. KW - Molecular biology. KW - Oncology. KW - Medicine & Public Health. KW - Molecular Medicine. KW - Biobanks KW - Health facilities KW - Procurement of organs, tissues, etc. KW - Tissue banks KW - Transplantation of organs, tissues, etc. KW - Oncology  . KW - Tumors KW - Clinical sciences KW - Medical profession KW - Human biology KW - Life sciences KW - Medical sciences KW - Physicians KW - Disease (Pathology) KW - Diseases KW - Medicine, Preventive KW - Health Workforce KW - Molecular biochemistry KW - Molecular biophysics KW - Biochemistry KW - Biophysics KW - Biomolecules KW - Systems biology UR - https://www.unicat.be/uniCat?func=search&query=sysid:7542662 AB - A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.   This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries. ER -