TY - BOOK ID - 7071596 TI - Capillary Electrophoresis of Proteins and Peptides AU - Strege, Mark A. AU - Lagu, Avinash L. PY - 2004 VL - 276 SN - 10643745 SN - 1588290174 159259798X 9786610358250 1280358254 9781592597987 9781588290175 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Capillary electrophoresis KW - Proteins KW - Peptides KW - Electrophoresis, Capillary KW - Separation KW - methods KW - analysis KW - Capillary electrophoresis -- Laboratory manuals. KW - Peptides -- Separation -- Laboratory manuals. KW - Proteins -- Separation -- Laboratory manuals. KW - Electrophoresis KW - Amino Acids, Peptides, and Proteins KW - Investigative Techniques KW - Chemistry Techniques, Analytical KW - Chemicals and Drugs KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Electrochemical Techniques KW - Methods KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Animal Biochemistry KW - Organic compounds KW - Amino acids KW - Proteids KW - Biomolecules KW - Polypeptides KW - Proteomics KW - Capillary gel electrophoresis KW - Capillary zone electrophoresis KW - Gel electrophoresis KW - Zone electrophoresis KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition KW - Capillary electrophoresis - Laboratory manuals KW - Proteins - Separation - Laboratory manuals KW - Peptides - Separation - Laboratory manuals KW - Electrophoresis, Capillary - methods KW - Proteins - analysis UR - https://www.unicat.be/uniCat?func=search&query=sysid:7071596 AB - Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development. ER -