TY - BOOK ID - 7058744 TI - In Situ Hybridization Protocols AU - Darby, Ian A. AU - Hewitson, Tim D. PY - 2006 VL - 326 SN - 10643745 SN - 1588294021 9781588294029 9786610358588 1280358580 1597450073 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - In situ hybridization KW - In Situ Hybridization KW - Laboratory manuals KW - Electronic books. -- local. KW - In situ hybridization -- Laboratory manuals. KW - Nucleic acid hybridization. KW - Laboratory Manuals KW - Staining and Labeling KW - Publication Formats KW - Nucleic Acid Hybridization KW - Publication Characteristics KW - Histocytological Preparation Techniques KW - Genetic Techniques KW - Investigative Techniques KW - Cytological Techniques KW - Histological Techniques KW - Clinical Laboratory Techniques KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Genetics KW - Biology KW - Health & Biological Sciences KW - Cytogenetics KW - Cytology KW - Hybridization KW - Nucleic acid hybridization KW - Technique KW - Biochemistry. KW - Cytology. KW - Biochemistry, general. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Cells KW - Cytologists KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Chemistry KW - Medical sciences KW - Composition KW - In situ hybridization - Laboratory manuals KW - In Situ Hybridization - laboratory manuals UR - https://www.unicat.be/uniCat?func=search&query=sysid:7058744 AB - In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully. ER -