TY - BOOK ID - 69085950 TI - Chimeric RNA : Methods and Protocols AU - Li, Hui. AU - Elfman, Justin. PY - 2020 SN - 1493999036 1493999044 PB - New York, NY : Springer US : Imprint: Humana, DB - UniCat KW - RNA KW - Àcid ribonucleic KW - ARN KW - Àcids nucleics KW - Micro RNAs KW - Genetics. KW - Medical genetics. KW - Cancer. KW - Genetics and Genomics. KW - Medical Genetics. KW - Cancer Biology. KW - Cancers KW - Carcinoma KW - Malignancy (Cancer) KW - Malignant tumors KW - Tumors KW - Clinical genetics KW - Diseases KW - Heredity of disease KW - Human genetics KW - Medical sciences KW - Pathology KW - Genetic disorders KW - Biology KW - Embryology KW - Mendel's law KW - Adaptation (Biology) KW - Breeding KW - Chromosomes KW - Heredity KW - Mutation (Biology) KW - Variation (Biology) KW - Genetic aspects UR - https://www.unicat.be/uniCat?func=search&query=sysid:69085950 AB - This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome. ER -