TY - BOOK ID - 69084848 TI - The ESCRT Complexes : Methods and Protocols AU - Culetto, Emmanuel. AU - Legouis, Renaud. PY - 2019 SN - 1493994913 1493994921 PB - New York, NY : Springer New York : Imprint: Humana, DB - UniCat KW - Cell membranes. KW - Biochemistry. KW - Cell physiology. KW - Membrane Biology. KW - Protein Science. KW - Cell Physiology. KW - Cell function KW - Cytology KW - Physiology KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Cell surfaces KW - Cytoplasmic membranes KW - Plasma membranes KW - Plasmalemma KW - Membranes (Biology) KW - Glycocalyces KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:69084848 AB - This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology. ER -