TY - BOOK ID - 6861249 TI - Receptor Signal Transduction Protocols PY - 1997 VL - 83 SN - 0896034186 089603495X 1280836857 1592595677 9780896034181 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Cellular signal transduction KW - Cell receptors KW - Research KW - Methodology KW - Physiology KW - Signal Transduction KW - Biochemical Processes KW - Cell Physiological Processes KW - Biological Science Disciplines KW - Cell Physiological Phenomena KW - Natural Science Disciplines KW - Biochemical Phenomena KW - Chemical Processes KW - Disciplines and Occupations KW - Chemical Phenomena KW - Phenomena and Processes KW - Cytology KW - Biology KW - Health & Biological Sciences KW - Methodology. KW - physiology. KW - Medicine. KW - Neurosciences. KW - Biomedicine. KW - Neural sciences KW - Neurological sciences KW - Neuroscience KW - Medical sciences KW - Nervous system KW - Clinical sciences KW - Medical profession KW - Human biology KW - Life sciences KW - Pathology KW - Physicians KW - Cellular signal transduction - Research - Methodology KW - Cell receptors - Research - Methodology UR - https://www.unicat.be/uniCat?func=search&query=sysid:6861249 AB - R. A. J. Challiss assembles in Receptor Signal Transduction Protocols an outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. These powerful methods emphasize the regulation of G-protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G-proteins, and approaches to studying how receptor expression and function is regulated. Designed to be easily reproducible, the protocols offered include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G-protein function, antisense methods for defining receptor-effector coupling pathways, and many more. Receptor Signal Transduction Protocols blends authoritative descriptions and applications of well-established methodologies with many novel techniques at the cutting edge of signal transduction research. The coverage cuts across disciplines and allows those already well-versed in one area to expand their repertoire of potent methods and broaden their research horizons. Every researcher interested in signal transduction today will find this richly practical guide indispensable. ER -