TY - BOOK ID - 64614260 TI - Nitric Oxide Protocols PY - 2004 SN - 1280359986 9786610359981 1592598072 1588292371 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:64614260 AB - Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects. ER -