TY - BOOK ID - 64248052 TI - Plant Synthetic Promoters : Methods and Protocols PY - 2016 SN - 1493963961 1493963945 PB - New York, NY : Springer New York : Imprint: Humana, DB - UniCat KW - Botany. KW - Plant Sciences. KW - Botanical science KW - Phytobiology KW - Phytography KW - Phytology KW - Plant biology KW - Plant science KW - Biology KW - Natural history KW - Plants KW - Floristic botany UR - https://www.unicat.be/uniCat?func=search&query=sysid:64248052 AB - This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function. ER -