TY - BOOK ID - 63986560 TI - Antiviral Resistance in Plants : Methods and Protocols AU - Watson, John M. AU - Wang, Ming-Bo. PY - 2012 SN - 1617798827 1617798819 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Botany. KW - Plant diseases. KW - Medical virology. KW - Plant Sciences. KW - Plant Pathology. KW - Virology. KW - Botanical science KW - Phytobiology KW - Phytography KW - Phytology KW - Plant biology KW - Plant science KW - Biology KW - Natural history KW - Plants KW - Medical microbiology KW - Virology KW - Virus diseases KW - Botany KW - Communicable diseases in plants KW - Crop diseases KW - Crops KW - Diseases of plants KW - Microbial diseases in plants KW - Pathological botany KW - Pathology, Vegetable KW - Phytopathology KW - Plant pathology KW - Vegetable pathology KW - Agricultural pests KW - Crop losses KW - Diseased plants KW - Phytopathogenic microorganisms KW - Plant pathologists KW - Plant quarantine KW - Pathology KW - Diseases and pests KW - Diseases KW - Wounds and injuries KW - Floristic botany UR - https://www.unicat.be/uniCat?func=search&query=sysid:63986560 AB - Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait. ER -