TY - BOOK ID - 63986189 TI - Fluorescence Spectroscopy and Microscopy : Methods and Protocols AU - Engelborghs, Yves. AU - Visser, Antonie J.W.G. PY - 2014 SN - 1627036490 1627036482 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Radiology, Medical. KW - Microscopy. KW - Spectroscopy. KW - Imaging / Radiology. KW - Biological Microscopy. KW - Spectroscopy/Spectrometry. KW - Clinical radiology KW - Radiology, Medical KW - Radiology (Medicine) KW - Medical physics KW - Analysis, Microscopic KW - Light microscopy KW - Micrographic analysis KW - Microscope and microscopy KW - Microscopic analysis KW - Optical microscopy KW - Optics KW - Analysis, Spectrum KW - Spectra KW - Spectrochemical analysis KW - Spectrochemistry KW - Spectroscopy KW - Chemistry, Analytic KW - Interferometry KW - Radiation KW - Wave-motion, Theory of KW - Absorption spectra KW - Light KW - Spectroscope KW - Qualitative KW - Spectrometry KW - Analytical chemistry UR - https://www.unicat.be/uniCat?func=search&query=sysid:63986189 AB - Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.   Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques. ER -