TY - BOOK ID - 63978881 TI - Bioconjugation Protocols : Strategies and Methods PY - 2011 SN - 1617791512 1617791504 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Biochemistry. KW - Biomaterials. KW - Biomedical engineering. KW - Biochemistry, general. KW - Biomedical Engineering and Bioengineering. KW - Biocompatible materials KW - Biomaterials KW - Medical materials KW - Medicine KW - Biomedical engineering KW - Materials KW - Biocompatibility KW - Prosthesis KW - Clinical engineering KW - Medical engineering KW - Bioengineering KW - Biophysics KW - Engineering KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition KW - Bioartificial materials KW - Hemocompatible materials KW - Biomaterials (Biomedical materials) UR - https://www.unicat.be/uniCat?func=search&query=sysid:63978881 AB - Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials. ER -