TY - BOOK ID - 63973819 TI - Genetic Modification of Hematopoietic Stem Cells : Methods and Protocols PY - 2009 SN - 1597454095 1588299805 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Biochemistry. KW - Cytology. KW - Stem cells. KW - Gene therapy. KW - Biochemistry, general. KW - Cell Biology. KW - Stem Cells. KW - Gene Therapy. KW - Cell biology KW - Cellular biology KW - Biology KW - Cells KW - Cytologists KW - Colony-forming units (Cells) KW - Mother cells KW - Progenitor cells KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Chemistry KW - Medical sciences KW - Therapy, Gene KW - Genetic engineering KW - Therapeutics KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:63973819 AB - With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. ER -