TY - BOOK ID - 5453312 TI - In Vitro Transcription and Translation Protocols AU - Tymms, Martin J AU - SpringerLink (Online service) PY - 1995 SN - 9780896032880 0896032884 9781592595242 9786610836475 1280836474 1592595243 1489940715 PB - Totowa, NJ Humana Press :Imprint: Humana DB - UniCat KW - Protein Biosynthesis KW - Transcription, Genetic KW - Genetic Techniques KW - Molecular Biology KW - Molecular genetics KW - Genetic transcription KW - Genetic translation KW - Génétique moléculaire KW - Transcription génétique KW - Traduction génétique KW - Laboratory Manuals KW - Laboratory manuals KW - Manuels de laboratoire KW - Moleuclar genetics KW - Peptide Biosynthesis KW - Biochemical Processes KW - Gene Expression KW - Biochemistry KW - Genetics KW - Investigative Techniques KW - Chemical Processes KW - Metabolism KW - Biology KW - Biological Science Disciplines KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Genetic Processes KW - Chemistry KW - Biochemical Phenomena KW - Chemical Phenomena KW - Natural Science Disciplines KW - Genetic Phenomena KW - Metabolic Phenomena KW - Disciplines and Occupations KW - Phenomena and Processes KW - Health & Biological Sciences KW - Laboratory manuals. KW - -Genetic transcription KW - -Genetic translation KW - -577.2 KW - 57.088 KW - Translation (Genetics) KW - Genetic code KW - Genetic regulation KW - Transcription (Genetics) KW - Molecular bases of life. Molecular biology KW - Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - 577.2 Molecular bases of life. Molecular biology KW - 577.2 KW - Life sciences. KW - Cell biology. KW - Life Sciences. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Cells KW - Cytologists KW - Biosciences KW - Sciences, Life KW - Science KW - Cytology. UR - https://www.unicat.be/uniCat?func=search&query=sysid:5453312 AB - Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important. ER -