TY - BOOK ID - 5347728 TI - RNA interference, editing, and modification : methods and protocols PY - 2004 VL - 265 SN - 1588292428 1592597750 1280360267 9786610360260 PB - Clifton, NJ : Humana Press, DB - UniCat KW - RNA Editing KW - RNA Interference KW - RNA editing KW - ARN KW - Laboratory manuals. KW - Edition KW - Manuels de laboratoire KW - RNA editing -- Laboratory manuals. KW - Laboratory Manuals KW - Publication Formats KW - RNA Processing, Post-Transcriptional KW - Gene Silencing KW - Epigenesis, Genetic KW - Biochemical Processes KW - Publication Characteristics KW - Metabolism KW - Gene Expression Regulation KW - Genetic Processes KW - Chemical Processes KW - Metabolic Phenomena KW - Biochemical Phenomena KW - Genetic Phenomena KW - Chemical Phenomena KW - Phenomena and Processes KW - Genetics KW - Biology - General KW - Biochemistry KW - Biology KW - Chemistry KW - Health & Biological Sciences KW - Physical Sciences & Mathematics KW - Editing, RNA KW - Messenger RNA editing KW - mRNA editing KW - Genetic regulation KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Medical sciences KW - Composition KW - Genetic regulation. KW - Genetic Phenomena. KW - Rna editing KW - Rna interference UR - https://www.unicat.be/uniCat?func=search&query=sysid:5347728 AB - The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to study these processes in most of the major biological systems in which they are known to occur-a wide range of organisms that includes worms, flies, trypanosomes, mammals, and plants. A historical overview of each subject is provided to put related chapters into a larger context. RNA interference (RNAi) has also emerged in recent years as an important and promising tool for gene discovery and reverse genetic engineering. Application of this cutting-edge technique is described for a range of experimental systems. Topics of interest include stable and transient RNA interference, RNA editing, gene silencing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs, many of which are involved in RNA interference or modification. In addition, techniques used to study RNA editing mechanisms are elaborated for both systems that involve the conversion of one base to another and insertion-deletion editing. To promote the adaptation of their assays and approaches for use in other biological systems, the authors have included a range of methods representative of the major experimental systems in a given field. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, RNA Interference, Editing, and Modification: Methods and Protocols offers not only geneticists, molecular biologists, and bioinformaticists a comprehensive collection of readily reproducible techniques for the elucidation of gene function and the alteration of gene expression, but also describes how best they may be productively used to address outstanding mechanistic questions and adapted to novel biological systems. ER -