TY - BOOK ID - 5343541 TI - cDNA Library Protocols AU - Cowell, Ian G. AU - Austin, Caroline A. PY - 1997 VL - 69 SN - 089603383X 9786610836765 1280836768 1592595553 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - DNA, Complementary KW - Gene Library KW - Genetic Techniques KW - Antisense DNA KW - DNA KW - Gene libraries KW - Molecular cloning KW - ADN KW - Clonage moléculaire KW - Laboratory manuals KW - Manuels de laboratoire KW - 57.088 KW - 577.212.3 KW - -DNA KW - -Gene libraries KW - -Molecular cloning KW - -#ABIB:aimm KW - Cloning, Molecular KW - DNA cloning KW - Gene cloning KW - Cloning KW - Genetic engineering KW - Molecular genetics KW - Clone cells KW - Gene banks (Genetic engineering) KW - Genomic banks KW - Genomic libraries KW - Biological resource centers KW - Genomes KW - Recombinant DNA KW - Deoxyribonucleic acid KW - Desoxyribonucleic acid KW - Thymonucleic acid KW - TNA (Nucleic acid) KW - Deoxyribose KW - Nucleic acids KW - Genes KW - Complementary DNA KW - Antisense nucleic acids KW - Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. KW - Antisense DNA -- Laboratory manuals. KW - DNA -- Laboratory manuals. KW - Electronic books. -- local. KW - Gene libraries -- Laboratory manuals. KW - Molecular cloning -- Laboratory manuals. KW - DNA, Single-Stranded KW - DNA Probes KW - Genetic Structures KW - Investigative Techniques KW - Nucleic Acid Probes KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Genetic Phenomena KW - Nucleic Acids KW - Phenomena and Processes KW - Molecular Probes KW - Laboratory Chemicals KW - Nucleic Acids, Nucleotides, and Nucleosides KW - Chemicals and Drugs KW - Specialty Uses of Chemicals KW - Chemical Actions and Uses KW - Genetics KW - Biology KW - Health & Biological Sciences KW - DNA, Complementary. KW - Gene Library. KW - Genetic Techniques. KW - Laboratory manuals. KW - 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. KW - 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods KW - Antisense dna KW - Dna KW - Dna, complementary. KW - Gene library. KW - Genetic techniques. KW - Clonage moléculaire KW - #ABIB:aimm KW - Nucleic acid base and sequence compositon. Experimental deciphering of genetic code KW - Cytology. KW - Immunology. KW - Cell Biology. KW - Immunobiology KW - Life sciences KW - Serology KW - Cell biology KW - Cellular biology KW - Cells KW - Cytologists UR - https://www.unicat.be/uniCat?func=search&query=sysid:5343541 AB - The first libraries of complementary DNA (cDNA) clones were con­ structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con­ structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we present a series of up-to-date protocols that together should give a good grounding of proce­ dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine up-to-date versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula­ tion, Chapters 6 and 7 describe means of cloning difficult-to-obtain ends of cDNAs, Chapters 8-18 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project. ER -