TY - BOOK ID - 5330908 TI - mRNA processing and metabolism : methods and protocols PY - 2004 VL - 257 SN - 1588292258 1592597505 128036047X 9786610360475 PB - Totowa, N.J. : Humana Press, DB - UniCat KW - Messenger RNA KW - ARN messager KW - Laboratory manuals KW - Manuels de laboratoire KW - Electronic books. -- local. KW - Genetics -- Technique. KW - Messenger RNA -- Laboratory manuals. KW - RNA, Messenger KW - Genetic Techniques KW - Metabolism KW - Metabolic Phenomena KW - RNA KW - Investigative Techniques KW - Nucleic Acids KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Phenomena and Processes KW - Nucleic Acids, Nucleotides, and Nucleosides KW - Chemicals and Drugs KW - Animal Biochemistry KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - 577.216 KW - 57.088.6 KW - Transfer of inheritance information. Transport of messenger RNA in the cell KW - Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. KW - 57.088.6 Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. KW - 577.216 Transfer of inheritance information. Transport of messenger RNA in the cell KW - Genetics KW - Technique. KW - Informational RNA KW - Messenger ribonucleic acid KW - mRNA KW - Protein transcript KW - Protein transcripts KW - Template RNA KW - Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques KW - Cytology. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Biology KW - Cells KW - Cytologists UR - https://www.unicat.be/uniCat?func=search&query=sysid:5330908 AB - mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research. ER -