TY - BOOK ID - 5297057 TI - DNA arrays : methods and protocols PY - 2001 VL - 170 SN - 089603822X 0585418969 1592592341 1280830379 9786610830374 1617372048 PB - Totowa, NJ : Humana Press, DB - UniCat KW - DNA microarrays KW - Puces à ADN KW - Laboratory manuals KW - Manuels de laboratoire KW - 577.21 KW - #WSCH:MODS KW - Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene KW - DNA microarrays -- Laboratory manuals. KW - DNA. KW - Electronic books. -- local. KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Animal Biochemistry KW - 577.21 Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene KW - Puces à ADN KW - Deoxyribonucleic acid KW - Desoxyribonucleic acid KW - Thymonucleic acid KW - TNA (Nucleic acid) KW - DNA biochips KW - Microarrays, DNA KW - Biochips KW - Immobilized nucleic acids KW - Deoxyribose KW - Nucleic acids KW - Genes KW - Cytology. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Biology KW - Cells KW - Cytologists UR - https://www.unicat.be/uniCat?func=search&query=sysid:5297057 AB - Microarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project. ER -