TY - BOOK ID - 4868742 TI - Targeted Genome Editing Using Site-Specific Nucleases : ZFNs, TALENs, and the CRISPR/Cas9 System PY - 2015 SN - 9784431552277 443155226X 9784431552260 4431552278 PB - Tokyo : Springer Japan : Imprint: Springer, DB - UniCat KW - Life Sciences. KW - Transgenics. KW - Developmental Biology. KW - Cell Biology. KW - Animal Genetics and Genomics. KW - Plant Genetics & Genomics. KW - Life sciences. KW - Cytology. KW - Developmental biology. KW - Plant breeding. KW - Animal genetics. KW - Transgenic organisms. KW - Sciences de la vie KW - Cytologie KW - Biologie du développement KW - Plantes KW - Génétique animale KW - Organismes génétiquement modifiés KW - Amélioration KW - Gene targeting KW - Genomes KW - Nucleases KW - Amino Acid Motifs KW - Investigative Techniques KW - Genetic Techniques KW - Genetic Structures KW - Gene Expression Regulation KW - Biology KW - Protein Structure, Secondary KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Genetic Processes KW - Biological Science Disciplines KW - Genetic Phenomena KW - Phenomena and Processes KW - Protein Conformation KW - Natural Science Disciplines KW - Disciplines and Occupations KW - Molecular Conformation KW - Molecular Structure KW - Biochemical Phenomena KW - Chemical Phenomena KW - Gene Targeting KW - Methods KW - Genetic Engineering KW - Genetics KW - Genome KW - Transcriptional Activation KW - Zinc Fingers KW - Health & Biological Sciences KW - Genomes. KW - Gene editing. KW - Editing, Gene KW - Editing, Genome KW - Genome editing KW - Cell biology. KW - Plant genetics. KW - Genomics KW - Haploidy KW - Plant Genetics and Genomics. KW - Plants KW - Cell biology KW - Cellular biology KW - Cells KW - Cytologists KW - Development (Biology) KW - Growth KW - Ontogeny KW - Genetically engineered organisms KW - Genetically modified organisms KW - GEOs (Genetically engineered organisms) KW - GMOs (Genetically modified organisms) KW - Organisms KW - Genetic engineering UR - https://www.unicat.be/uniCat?func=search&query=sysid:4868742 AB - This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields. ER -