TY - BOOK ID - 33239511 TI - Flow Cytometry Basics for the Non-Expert AU - Goetz, Christine. AU - Hammerbeck, Christopher. AU - Bonnevier, Jody. PY - 2018 SN - 3319980718 331998070X PB - Cham : Springer International Publishing : Imprint: Springer, DB - UniCat KW - Flow cytometry. KW - Cell counting, Laser KW - Cell separation, Laser KW - Cell sorting, Laser KW - Flow cytofluorometry KW - Flow cytophotometry KW - Laser cell counting KW - Laser cell separation KW - Laser cell sorting KW - Cytometry KW - Monoclonal antibodies. KW - Medical laboratories. KW - Antibodies. KW - Laboratory Medicine. KW - Diagnosis, Laboratory KW - Health facilities KW - Laboratories KW - Antibodies, Monoclonal KW - Monoclonal immunoglobulins KW - Immunoglobulins KW - Molecular cloning KW - Laboratory medicine. KW - Clinical medicine KW - Clinical pathology KW - Diagnostic laboratory tests KW - Laboratory diagnosis KW - Laboratory medicine KW - Medical laboratory diagnosis KW - Diagnosis KW - Pathology KW - Antibodies KW - Immune globulins KW - Immune serum globulin KW - Blood proteins KW - Globulins KW - Plasma cells KW - Antibody diversity KW - Antigens KW - Bacterial immunoglobulin-binding proteins UR - https://www.unicat.be/uniCat?func=search&query=sysid:33239511 AB - Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation. ER -