TY - BOOK ID - 18431186 TI - Microbial Gene Essentiality: Protocols and Bioinformatics AU - Osterman, Andrei L. AU - Gerdes, Svetlana Y. PY - 2008 SN - 1588293785 9781588293787 1597453218 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Computational Biology KW - Computational biology. KW - Genes, Essential KW - Genetic Techniques KW - Genetics, Microbial KW - Genome, Bacterial KW - Genomics KW - Microbial genetics. KW - Microbiological Techniques KW - genetics KW - methods KW - Microbial genetics KW - Computational biology KW - Genetics KW - Laboratory Manuals KW - Methods KW - Investigative Techniques KW - Genome KW - Biology KW - Clinical Laboratory Techniques KW - Microbiology KW - Publication Formats KW - Genes KW - Genetic Structures KW - Genome Components KW - Biological Science Disciplines KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Publication Characteristics KW - Natural Science Disciplines KW - Genetic Phenomena KW - Disciplines and Occupations KW - Phenomena and Processes KW - Health & Biological Sciences KW - Natural Sciences KW - Physical Sciences KW - Discipline, Natural Science KW - Disciplines, Natural Science KW - Natural Science KW - Natural Science Discipline KW - Physical Science KW - Science, Natural KW - Science, Physical KW - Sciences, Natural KW - Sciences, Physical KW - Biologic Sciences KW - Biological Science KW - Science, Biological KW - Sciences, Biological KW - Biological Sciences KW - Life Sciences KW - Biologic Science KW - Biological Science Discipline KW - Discipline, Biological Science KW - Disciplines, Biological Science KW - Life Science KW - Science Discipline, Biological KW - Science Disciplines, Biological KW - Science, Biologic KW - Science, Life KW - Sciences, Biologic KW - Sciences, Life KW - Component, Genome KW - Components, Genome KW - Genome Component KW - Genetic Structure KW - Structure, Genetic KW - Structures, Genetic KW - Cistron KW - Gene KW - Genetic Materials KW - Cistrons KW - Genetic Material KW - Material, Genetic KW - Materials, Genetic KW - Diagnoses and Laboratory Examinations KW - Diagnosis, Laboratory KW - Laboratory Diagnosis KW - Laboratory Examinations and Diagnoses KW - Laboratory Techniques, Clinical KW - Clinical Laboratory Technique KW - Diagnoses, Laboratory KW - Laboratory Diagnoses KW - Laboratory Technique, Clinical KW - Technique, Clinical Laboratory KW - Techniques, Clinical Laboratory KW - Genomes KW - Investigative Technics KW - Investigative Technic KW - Investigative Technique KW - Technic, Investigative KW - Technics, Investigative KW - Technique, Investigative KW - Techniques, Investigative KW - Methodological Studies KW - Methodological Study KW - Procedures KW - Studies, Methodological KW - Study, Methodological KW - Method KW - Procedure KW - Genetic Technic KW - Genetic Technics KW - Genetic Technique KW - Technic, Genetic KW - Technics, Genetic KW - Technique, Genetic KW - Techniques, Genetic KW - Microbiologic Technic KW - Microbiologic Technics KW - Microbiologic Technique KW - Microbiological Technics KW - Technic, Microbiologic KW - Technics, Microbiological KW - Technique, Microbiologic KW - Techniques, Microbiologic KW - Microbiologic Techniques KW - Microbiological Technic KW - Microbiological Technique KW - Technic, Microbiological KW - Technics, Microbiologic KW - Technique, Microbiological KW - Techniques, Microbiological KW - Bio-Informatics KW - Biology, Computational KW - Computational Molecular Biology KW - Bioinformatics KW - Molecular Biology, Computational KW - Bio Informatics KW - Bio-Informatic KW - Bioinformatic KW - Biologies, Computational Molecular KW - Biology, Computational Molecular KW - Computational Molecular Biologies KW - Molecular Biologies, Computational KW - Microbial Genetics KW - Genetic, Microbial KW - Microbial Genetic KW - Bacterial Genomes KW - Genomes, Bacterial KW - Bacterial Genome KW - Microorganisms KW - Genetic Concepts KW - Genetic Phenomenon KW - Genetic Process KW - Genetic Processes KW - Concept, Genetic KW - Concepts, Genetic KW - Genetic Concept KW - Phenomena, Genetic KW - Phenomenon, Genetic KW - Process, Genetic KW - Processes, Genetic KW - Genes, House-keeping KW - Genes, Housekeeping KW - Essential Gene KW - Essential Genes KW - Gene, Essential KW - Gene, House-keeping KW - Gene, Housekeeping KW - Genes, House keeping KW - House-keeping Gene KW - House-keeping Genes KW - Housekeeping Gene KW - Housekeeping Genes KW - Biochemistry. KW - Cytology. KW - Human genetics. KW - Biochemistry, general. KW - Cell Biology. KW - Human Genetics. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Chemistry KW - Medical sciences KW - Heredity, Human KW - Human biology KW - Physical anthropology KW - Cell biology KW - Cellular biology KW - Cells KW - Cytologists KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:18431186 AB - Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists. ER -