TY - BOOK ID - 17945167 TI - Calpain Methods and Protocols PY - 2000 SN - 0896036324 9786610820429 128082042X 1592590500 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Calpain KW - Cysteine proteinases. KW - Proteinase KW - Calcium-dependent cysteine proteinase KW - Cysteine proteinases KW - Neurosciences. KW - Neural sciences KW - Neurological sciences KW - Neuroscience KW - Medical sciences KW - Nervous system UR - https://www.unicat.be/uniCat?func=search&query=sysid:17945167 AB - In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of ยต- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains. ER -