TY - BOOK ID - 17907250 TI - Immunocytochemical methods and protocols PY - 1999 SN - 0896035700 0896038130 0585246181 1280830212 1592592139 1281115959 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Immunocytochemistry KW - In situ hybridization KW - Nucleic acid hybridization KW - Cytochemistry KW - Immunochemistry KW - Immunofluorescence KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:17907250 AB - In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases. ER -