TY - BOOK ID - 17893437 TI - Phospholipid signaling protocols PY - 1998 SN - 0896034917 0585242208 9786610830480 1280830484 1592592554 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Cellular signal transduction KW - Membrane lipids KW - Phospholipids KW - Second messengers (Biochemistry) KW - Research KW - Methodology. KW - Second messenger systems (Biochemistry) KW - Secondary messengers (Biochemistry) KW - Phosphatides KW - Phosphates KW - Membranes (Biology) KW - Lipids KW - Cellular information transduction KW - Information transduction, Cellular KW - Signal transduction, Cellular KW - Bioenergetics KW - Cellular control mechanisms KW - Information theory in biology KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:17893437 AB - In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry. Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field. ER -