TY - BOOK ID - 17140948 TI - Signal Transduction Protocols AU - Dickson, Robert C. AU - Mendenhall, Michael D. PY - 2004 VL - 284 SN - 1588292452 1592598161 9786610359899 1280359897 9781592598168 9781588292452 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Cellular signal transduction KW - G proteins KW - Cell receptors KW - GTP-Binding Proteins KW - Signal Transduction KW - analysis KW - physiology KW - Cell membrane receptors KW - Cell surface receptors KW - Receptors, Cell KW - Binding sites (Biochemistry) KW - Cell membranes KW - Proteins KW - GTP-binding proteins KW - GTP regulatory proteins KW - Guanine nucleotide-binding proteins KW - Guanine nucleotide regulatory proteins KW - Membrane proteins KW - Cellular information transduction KW - Information transduction, Cellular KW - Signal transduction, Cellular KW - Bioenergetics KW - Cellular control mechanisms KW - Information theory in biology KW - Cytology. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Biology KW - Cells KW - Cytologists KW - Cellular signal transduction - Laboratory manuals KW - G proteins - Laboratory manuals KW - Cell receptors - Laboratory manuals KW - GTP-Binding Proteins - analysis - laboratory manuals KW - Signal Transduction - physiology - Laboratory Manuals UR - https://www.unicat.be/uniCat?func=search&query=sysid:17140948 AB - Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach. ER -