TY - BOOK ID - 17105604 TI - Protein engineering protocols AU - Arndt, Katja M. AU - Müller, Kristian M. PY - 2007 VL - 352 SN - 1588290727 9781588290724 9786610832576 1280832576 1597451878 PB - Totowa, N.J. : Humana Press, DB - UniCat KW - Protein engineering KW - Protein Engineering KW - methods KW - Protein engineering. KW - Bio-process engineering KW - Bioprocess engineering KW - Engineering, Protein KW - Protein design KW - Biochemical engineering. KW - Proteins KW - Biochemical engineering KW - Genetic engineering KW - Biochemistry KW - Biotechnology KW - Chemical engineering KW - Design KW - Biochemistry. KW - Cytology. KW - Medicine. KW - Protein Science. KW - Cell Biology. KW - Molecular Medicine. KW - Biochemistry, general. KW - Health Workforce KW - Cell biology KW - Cellular biology KW - Biology KW - Cells KW - Cytologists KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Chemistry KW - Medical sciences KW - Composition KW - Protein Engineering - methods - Laboratory Manuals UR - https://www.unicat.be/uniCat?func=search&query=sysid:17105604 AB - Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented. Following the successful Methods in Molecular Biology™ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators. ER -