TY - BOOK ID - 15936295 TI - Protein stability and folding : theory and practice PY - 1995 SN - 0896033015 9786610836499 1280836490 1592595278 PB - Totowa Humana press DB - UniCat KW - Protein folding KW - Proteins KW - -Folding of proteins KW - Proteids KW - Biomolecules KW - Polypeptides KW - Proteomics KW - Conformation KW - Folding KW - Protein folding. KW - Conformation. KW - Protein conformation KW - Folding of proteins KW - Biochemistry. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Composition KW - PROTEINS KW - PROTEIN FOLDING KW - CONFORMATION KW - PROPERTIES UR - https://www.unicat.be/uniCat?func=search&query=sysid:15936295 AB - The intent of this work is to bring together in a single volume the techniques that are most widely used in the study of protein stability and protein folding. Over the last decade our understanding of how p- teins fold and what makes the folded conformation stable has advanced rapidly. The development of recombinant DNA techniques has made possible the production of large quantities of virtually any protein, as well as the production of proteins with altered amino acid sequence. Improvements in instrumentation, and the development and refinement of new techniques for studying these recombinant proteins, has been central to the progress made in this field. To give the reader adequate background information about the s- ject, the first two chapters of this book review two different, yet related, aspects of protein stability. The first chapter presents a review of our current understanding of the forces involved in determining the conf- mational stability of proteins as well as their three-dimensional folds. The second chapter deals with the chemical stability of proteins and the pathways by which their covalent structure can degrade. The remainder of the book is devoted to techniques used in the study of these two major areas of protein stability, as well as several areas of active research. Although some techniques, such as X-ray crystallography and mass spectroscopy, are used in the study of protein stability, they are beyond the scope of this book and will not be covered extensively. ER -