TY - BOOK ID - 14302286 TI - Multistate GTPase control co-translational protein targeting : doctoral thesis for chemistry accepted by California Institute of Technology, Pasadena, CA, USA PY - 2012 SN - 1441978070 9786613350770 1283350777 1441978089 PB - New York : Springer, DB - UniCat KW - Guanosine triphosphatase. KW - Protein binding. KW - Chemistry KW - Human Anatomy & Physiology KW - Physical Sciences & Mathematics KW - Health & Biological Sciences KW - Organic Chemistry KW - Animal Biochemistry KW - G proteins KW - Guanosine triphosphatase KW - Biological transport. KW - Physiological transport. KW - GTP-binding proteins KW - GTP regulatory proteins KW - Guanine nucleotide-binding proteins KW - Guanine nucleotide regulatory proteins KW - Membrane transport KW - Passive transport, Biological KW - Physiological transport KW - Transport, Biological KW - GTP phosphohydrolase KW - GTPase KW - Guanosine triphosphate phosphohydrolase KW - Guanosinetriphosphatase KW - Chemistry. KW - Analytical chemistry. KW - Bioorganic chemistry. KW - Biochemistry. KW - Proteomics. KW - Bioorganic Chemistry. KW - Analytical Chemistry. KW - Biochemistry, general. KW - Molecular biology KW - Proteins KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Medical sciences KW - Bio-organic chemistry KW - Biological organic chemistry KW - Biochemistry KW - Chemistry, Organic KW - Analysis, Chemical KW - Analytical chemistry KW - Chemical analysis KW - Metallurgical analysis KW - Mineralogy, Determinative KW - Physical sciences KW - Composition KW - Diffusion KW - Osmosis KW - Phosphatases KW - Membrane proteins KW - Analytical biochemistry. KW - Analytic biochemistry KW - Chemistry, Analytic KW - Bioanalytic chemistry KW - Bioanalytical chemistry KW - Analytic chemistry UR - https://www.unicat.be/uniCat?func=search&query=sysid:14302286 AB - Proteins act as macromolecular machinery that mediate many diverse biological processes - the molecular mechanisms of this machinery has fascinated biologists for decades. Analysis of the kinetic and thermodynamic features of these mechanisms could reveal unprecedented aspects of how the machinery function and will eventually lead to a novel understanding of various biological processes. This dissertation comprehensively demonstrates how two universally conserved guanosine triphosphatases in the signal recognition particle and its membrane receptor maintain the efficiency and fidelity of the co-translational protein targeting process essential to all cells. A series of quantitative experiments reveal that the highly ordered and coordinated conformational states of the machinery are the key to their regulatory function. This dissertation also offers a mechanistic view of another fascinating system in which multistate protein machinery closely control critical biological processes. ER -