TY - BOOK ID - 138225272 TI - Sample Preparation in Metabolomics AU - Kuligowski, Julia AU - Quintás, Guillermo PY - 2021 PB - Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute DB - UniCat KW - metabolomics KW - sample preparation KW - hydrophilic interaction liquid chromatography KW - ion mobility spectrometry KW - high resolution mass spectrometry KW - design of experiments KW - AMOPLS KW - metabonomics KW - metabolic profiling KW - NMR KW - nuclear magnetic resonance spectroscopy KW - cell line KW - human cell line KW - MiaPaCa-2 KW - Panc-1 KW - AsPC-1 KW - extracellular vesicles KW - exosomes KW - microvesicles KW - biomarkers KW - diagnostics KW - metabolic pathways KW - plant metabolomics KW - forestry KW - trees KW - mass spectrometry KW - metabolite extraction KW - GC-MS KW - LC-MS KW - metadata standardization KW - databases KW - multicellular tumor spheroids KW - metallodrugs KW - oxaliplatin KW - KP1339 KW - method development KW - IT-139 KW - 20% FCS KW - harvesting KW - extraction KW - metabolites KW - normalization KW - electromembrane extraction KW - cardiovascular disease KW - multi-segment injection KW - capillary electrophoresis–mass spectrometry KW - liquid chromatography–mass spectrometry KW - plant natural products KW - drug discovery KW - liquid chromatography KW - gas chromatography KW - human milk KW - metabolome KW - sampling KW - liquid chromatography–mass spectrometry (LC-MS) KW - nuclear magnetic resonance (NMR) KW - gas chromatography–mass spectrometry (GC-MS) KW - capillary electrophoresis—mass spectrometry (CE-MS) KW - high-resolution magic angle spinning KW - microscopic samples KW - lipidomics KW - LC-MS/MS KW - human plasma UR - https://www.unicat.be/uniCat?func=search&query=sysid:138225272 AB - Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees. ER -